1株乙羧氟草醚降解菌的分离鉴定与降解特性研究

本研究从某农药厂污水处理池的活性污泥中分离得到1株能以乙羧氟草醚为唯一碳源生长的菌株。经生理生化鉴定和16SrRNA基因序列同源性分析，将此菌株初步鉴定为腐生葡萄球茵（Staphylococcussaprophyticus），并命名为YSC．1。对菌株YSC．1的生长特性研究表明：茵株的最佳生长温度和pH分别为30℃、7．0；NaCI浓度对菌株YSC-1生长有较大的影响。菌株在20℃-40℃之间均能降解乙羧氟草醚，在30％1、pH7．0的条件下对乙羧氟草醚的降解率最高；增加乙羧氟草醚的浓度会对菌株产生毒害作用，降低其降解率；提高接种量可以加快乙羧氟草醚的降解。在乙羧氟草醚终浓度为100mg／L的工业废水经7d处理后，乙羧氟草醚的去除率达91．62％，说明菌株YSC-1在废水处理中具有很好的应用前景。

Isolation and Identification of a Strain of Fluoroglycofen Degradation Bacteria and Its Degradation Research

In this study, a bacterial strain using fluoroglycofen as sole carbon source was isolated from activated sludge of wastewater treatment tank in an insecticide factory. It was identified by physiological-biochemical identification and 16S rRNA gene sequences homology analysis, it belonged to Staphylococcus saprophyticus. The strain was named as YSC-1 and its growth was studied. The results showed that the optimum growth conditions for YSC-1 were 30℃ and pI-I 7. 0. The concentration of Nacl affected the growth significantly. The strain degraded fluoroglycofen at temperature of 20℃ -40℃ with the maximum fluoroglyoofen degrading rate at 30℃ and 7. 0, respectively. Increasing concentration of tluoroglycofen resulted in toxic effect on strain YSC-lto reduced the biodegradation rate. Increasing inoculums volume would speed up the degradation of tluoroglycofen. For industrial wastewater treated for seven days with final fluoroglycofen concentration of 100 mg/L, the removal rate reached 91.62%, which showed that the strain YSC-1 had a good application prospect for treatment of wastcwater.