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Effect of inoculum to substrate ratio (I/S) on municipal solid waste anaerobic degradation kinetics and potential
Authors:Anthony Boulanger  Elisabeth Pinet  Marielle Bouix  Théodore Bouchez  Alicia A Mansour
Institution:1. CEMAGREF, Hydrosystems and Bioprocesses Unit, Parc de Tourvoie, 92163 Antony, France;2. Veolia Environnement Recherche et Innovation (VERI), 291 Avenue Dreyfous Ducas, Zone Portuaire de Limay, 78520 Limay, France;3. AgroParisTech, UMR Génie et Microbiologie des Procédés Alimentaires, 78850 Grignon, France;1. Environmental Technology Division, School of Industrial Technology, Universiti Sains Malaysia, 11800 Penang, Malaysia;2. School of Chemical Engineering, Universiti Sains Malaysia, Engineering Campus, 14300 Nibong Tebal, Penang, Malaysia;3. Bioresource, Paper and Coatings Technology Division, School of Industrial Technology, Universiti Sains Malaysia, 11800 Penang, Malaysia;1. Key Laboratory of Environmental and Applied Microbiology, Environmental Microbiology Key Laboratory of Sichuan Province, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China;2. Key Laboratory of Development and Application of Rural Renewable Energy, Ministry of Agriculture, Biogas Institute of Ministry of Agriculture, Chengdu 610041, China;3. School of Life Science and Engineering, Lanzhou University of Technology, Lanzhou 730050, China
Abstract:The goal of this study is to evaluate the impact of the inoculum to substrate ratio (I/S) on the anaerobic degradation potential of municipal solid waste (MSW). Reconstituted MSW samples were thus incubated under batch anaerobic conditions and inoculated with an increasing amount of inoculum originating from a mesophilic sludge digester. I/S tested values were 0 (no inoculum added), 0.015, 0.03, 0.06, 0.12, 0.25, 1, 2 and 4 (gVMinoculum/gVMwaste). The results indicate that the apparent maximal rate of dissolved organic carbon accumulation is reached at I/S = 0.12. Under this level, the hydrolysis process is limited by the concentration of biomass and can thus be described as first order kinetics phenomena with respect to biomass for I/S ratios below 0.12. The maximum methane production rate and the minimal latency are reached at a ratio of 2. In addition to that, both methane signature and ARISA show a shift in the methanogenic populations from hydrogenotrophic to acetoclastic.
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