| 宁波三江口水域原核生物群落结构分析 |
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| 引用本文： | 胡安谊,李姜维,杨晓永,王弘杰,于昌平. 宁波三江口水域原核生物群落结构分析[J]. 环境科学, 2015, 36(7): 2487-2495 |
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| 作者姓名： | 胡安谊 李姜维 杨晓永 王弘杰 于昌平 |
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| 作者单位： | 1. 中国科学院城市环境研究所，城市污染物转化重点实验室，厦门 361021; 中国科学院宁波城市环境观测研究站，宁波 315800
2. 中国科学院城市环境研究所，城市污染物转化重点实验室，厦门 361021; 中国科学院宁波城市环境观测研究站，宁波 315800; 中国科学院大学，北京 100049 |
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| 基金项目： | 国家自然科学基金项目(41106096);宁波市科技计划项目(2013A610174, 2012C5011);中国科学院知识创新工程青年人才领域前沿项目(IUEQN201307) |
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| 摘 要： | 首次应用16S rRNA基因-Illumina Miseq高通量测序技术对甬江流域宁波三江口区域的表层水体原核生物群落进行了分析,共获得215 504条高质量序列.多样性指数分析表明,该流域的水体原核生物群落具有较高的遗传多样性和丰富度.菌群分类分析发现,β-变形菌纲(β-Proteobacterium)、放线菌门(Actinobacteria)和拟杆菌门(Bacteroidetes)是主要的优势菌群,其相对丰度占总群落的78.88%.对比不同站位的原核生物群落,发现水文环境可能是影响三江口水域原核生物群落组成和结构的重要因素之一.与以往报道对比分析表明,甬江流域分布有多种污水和粪便污染指示菌,其中余姚江污染指示菌的丰度最高,说明其受污染的风险可能较高.病原菌BLASTN分析表明,在种和亚种水平上,分别检出76和18种潜在病原菌,占序列总量的2.19%和0.40%.本研究为系统认识甬江流域原核生物群落结构及生态功能提供了重要基础数据.
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| 关 键 词： | 甬江流域 原核生物群落 16S rRNA基因-Illumina Miseq 测序 污染指示菌 潜在病原菌 生物信息分析 |
| 收稿时间： | 2014-12-12 |
| 修稿时间： | 2015-02-02 |
Analysis of Prokaryotic Community Structure in River Waters of the Ningbo Sanjiang Mouth |
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| HU An-yi,LI Jiang-wei,YANG Xiao-yong,WANG Hong-jie and YU Chang-ping. Analysis of Prokaryotic Community Structure in River Waters of the Ningbo Sanjiang Mouth[J]. Chinese Journal of Environmental Science, 2015, 36(7): 2487-2495 |
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| Authors: | HU An-yi LI Jiang-wei YANG Xiao-yong WANG Hong-jie YU Chang-ping |
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| Affiliation: | Key Laboratory of Urban Pollutant Conversion, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen 361021, China;Ningbo Urban Environment Observation and Research Station, Chinese Academy of Sciences, Ningbo 315800, China;Key Laboratory of Urban Pollutant Conversion, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen 361021, China;Ningbo Urban Environment Observation and Research Station, Chinese Academy of Sciences, Ningbo 315800, China;Key Laboratory of Urban Pollutant Conversion, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen 361021, China;Ningbo Urban Environment Observation and Research Station, Chinese Academy of Sciences, Ningbo 315800, China;Key Laboratory of Urban Pollutant Conversion, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen 361021, China;Ningbo Urban Environment Observation and Research Station, Chinese Academy of Sciences, Ningbo 315800, China;University of Chinese Academy of Sciences, Beijing 100049, China;Key Laboratory of Urban Pollutant Conversion, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen 361021, China;Ningbo Urban Environment Observation and Research Station, Chinese Academy of Sciences, Ningbo 315800, China |
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| Abstract: | |
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| Keywords: | Yongjiang River Watershed prokaryotic community 16S rRNA gene based-Illumina Miseq sequencing pollution indicator bacteria potential pathogenic bacteria bioinformatics analysis |
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