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High serum S-equol content in red clover fed ewes: the classical endocrine disruptor is a single enantiomer |
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| Authors: | Eeva A Mustonen Tuija Jokela Ilkka Saastamoinen Juhani Taponen Suvi Taponen Hannu Saloniemi Kristiina Wähälä |
| Institution: | (1) Saari Unit, Department of Clinical Veterinary Sciences, Faculty of Veterinary Medicine, University of Helsinki, Pohjoinen pikatie 800, FI-04920 Saarentaus, Finland;(2) Laboratory of Organic Chemistry, Department of Chemistry, Faculty of Science, University of Helsinki, P.O. Box 55, FIN-00014 Helsinki, Finland;(3) Section of Animal Hygiene, Department of Clinical Veterinary Sciences, Faculty of Veterinary Medicine, University of Helsinki, P.O. Box 57, FIN-00014 Helsinki, Finland |
| Abstract: | An expedient high performance liquid chromatography (HPLC) method was developed for the quantitative analysis of environmental estrogenic isoflavonoids, particularly (S)-equol, in red clover fed ewes. We report here the phytoestrogen analysis of red clover silage and the serum of ewes fed with this feed. Ewes received daily 10.5 g of formononetin on average from red clover silage. In the serum samples a major part of formononetin had been metabolised to equol (average 7.7 μg ml−1). According to chiral HPLC analysis, the equol present in the serum of ewes was solely of the (S)-form. We also demonstrate the presence of the metabolite O-demethylangolensin (O-DMA) in ovine serum. |
| Keywords: | Environmental estrogens Endocrine disruptors S-equol O-demethylangolensin Formononetin Sheep Serum Chiral HPLC HPLC Fluorescence detector |
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