Single walled carbon nanotube reactivity and cytotoxicity following extended aqueous exposure |
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Authors: | Barbara J Panessa-Warren Mathew M Maye Kenya M Crosson |
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Institution: | a Department of Energy Sciences and Technology, Brookhaven National Laboratory, 12 S Upton, P.O. Box 5000, Upton, NY 11973, USA b Center for Functional Nanomaterials, Brookhaven National Laboratory, P.O. Box 5000, Upton, NY 11973, USA c Instrumentation Division and the Center for Functional Nanomaterials, Brookhaven National Laboratory, P.O. Box 5000, Upton, NY 11973, USA d Department of Civil and Environmental Engineering & Mechanics, 300 College Park, University of Dayton, Dayton, OH 45469, USA |
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Abstract: | Globally carbon nanoparticles are increasingly utilized, yet it is not known if these nanoparticles pose a threat to the environment or human health. This investigation examined ‘as-prepared’, and acid cleaned carbon nanoparticle physicochemical characteristics (by FTIR, TEM, FESEM, UV-VIS and X-ray microanalysis), and whether these characteristics changed following 2.5-7 yr exposure to pH neutral saline or fresh water. To determine if these aqueous aged nanotubes were cytotoxic, these nanotubes were incubated with human epithelial monolayers and analyzed for cell viability (vital staining) and ultrastructural nanoparticle binding/localization (TEM, FESEM). The presence of Ni and Y catalyst, was less damaging to cells than CNT lattice surface oxidation. Extended fresh water storage of oxidized CNTs did not reduce surface reactive groups, nor lessen cell membrane destruction or cell death. However storing oxidized CNTs in saline or NOM significantly reduced CNT-induced cell membrane damage and increased cell survival to control levels. |
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Keywords: | Carbon nanotubes Cytotoxicity Human lung cells FTIR Carbon lattice oxidation NOM |
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