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模拟升温和放牧对高寒草甸土壤微生物群落的影响
引用本文:王蓓,孙庚,罗鹏,王志远,张艳博,吴宁,罗光荣.模拟升温和放牧对高寒草甸土壤微生物群落的影响[J].应用与环境生物学报,2011(2):151-158.
作者姓名:王蓓  孙庚  罗鹏  王志远  张艳博  吴宁  罗光荣
作者单位:中国科学院成都生物研究所生态恢复重点实验室;中国科学院研究生院;四川省龙日种畜场;
基金项目:中国科学院战略先导科技专项(No.XDA05050304); 国家“十一五”科技支撑计划项目(No.2007BAC18B02); 中国科学院“西部之光”人才培养计划一般项目; 西部博士资助项目及中国科学院山地生态恢复与生物资源利用重点实验室开放课题资助~~
摘    要:通过开顶式温室(Open top chambers,OTCs)升温以及刈割+施加牛粪处理,应用磷脂脂肪酸(Phospholipid fatty acids,PLFAs)方法,研究了青藏高原东部高寒草甸土壤微生物群落结构对气候变暖和放牧的响应.结果表明,高寒草甸在生长季节,微生物群落以细菌为主.平均1.17℃的土壤升温使土壤微生物PLFAs总量增加34.58%,而春季割草结合牛粪施加使微生物PLFAs总量增加65.77%.模拟变暖和放牧均引起土壤微生物群落结构的显著变化.升温使细菌相对含量增加8.80%,而使真菌相对含量降低17.48%,细菌与真菌之比由7.3变为9.6.放牧使细菌相对含量增加8.40%,真菌相对含量降低14.04%,细菌与真菌之比由7.3变为9.2.OTCs升温+放牧处理比单独的升温或放牧处理对土壤微生物总量和细菌与真菌比值的影响更加明显.本研究表明,气候变暖和人类活动能够在短期内显著地改变青藏高原高寒草甸土壤微生物群落结构,进而可能影响这一地区的生态系统碳收支和养分循环.

关 键 词:土壤微生物  群落结构  磷脂脂肪酸  模拟升温  气候变暖  放牧  牛粪  青藏高原  高寒草甸

Microbial Communities of Alpine Meadow Soil in the Eastern Qinghai-Tibetan Plateau Subjected to Experimental Warming and Grazing
WANG Bei,SUN Geng,LUO Peng,WANG Zhiyuan,ZHANG Yanbo,WU Ning & LUO Guangrong.Microbial Communities of Alpine Meadow Soil in the Eastern Qinghai-Tibetan Plateau Subjected to Experimental Warming and Grazing[J].Chinese Journal of Applied and Environmental Biology,2011(2):151-158.
Authors:WANG Bei    SUN Geng  LUO Peng  WANG Zhiyuan  ZHANG Yanbo  WU Ning & LUO Guangrong
Institution:WANG Bei1,2,SUN Geng1,LUO Peng1,WANG Zhiyuan1,ZHANG Yanbo1,WU Ning1 & LUO Guangrong3(1ECORES Lab,Chengdu Institute of Biology,Chinese Academy of Sciences,Chengdu 610041,China)(2Graduate University of Chinese Academy of Sciences,Beijing 100049,China)(3Longri Pedigree Station of Sichuan,Aba 624401,Sichuan,China)
Abstract:oil microbes are important for many underground ecological processes and the change in their total biomass and community structure is regarded as a sensitive indicator for climate warming and human disturbances.Through open top chambers(OTCs) warming and clipping plus dung application,the effects of warming and grazing on soil microbial biomass and community structure were determined in an alpine meadow on the eastern Qinghai-Tibetan Plateau.The profiles of microbial phospholipid fatty acids(PLFAs) showed t...
Keywords:soil microbe  community structure  phospholipid fatty acids(PLFAs)  experimental warming  climate warming  grazing  dung  Qinghai-Tibetan Plateau  alpine meadow  
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