| PCR-DGGE技术解析针叶和阔叶凋落物混合分解对土壤微生物群落结构的影响 |
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| 引用本文： | 陈法霖,张凯,郑华,林学强,欧阳志云,屠乃美.PCR-DGGE技术解析针叶和阔叶凋落物混合分解对土壤微生物群落结构的影响[J].应用与环境生物学报,2011(2):145-151. |
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| 作者姓名： | 陈法霖 张凯 郑华 林学强 欧阳志云 屠乃美 |
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| 作者单位： | 中国科学院生态环境研究中心城市与区域生态国家重点实验室;海南省环境科学研究院;湖南农业大学农学院; |
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| 基金项目： | 国家自然科学基金项目(Nos.40871130,30600474)资助~~ |
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| 摘 要： | 为深入理解凋落物类型和微生物群落之间的相互关系,通过小盆+凋落袋控制实验,运用PCR-DGGE技术解析了南方红壤丘陵区典型针叶树种马尾松和湿地松的凋落物分别与白栎、青冈两个阔叶树种凋落物混合分解对土壤微生物基因型多样性的影响.结果表明:1)针叶凋落物中加入阔叶后细菌群落结构未发生显著变化,将所有处理归为一类的相似度达72%,其差异仅表现在马尾松+白栎和湿地松+青冈处理土壤微生物群落16S rDNA的丰富度和多样性分别显著高于单一马尾松和湿地松;2)针叶凋落物中加入阔叶后真菌群落结构发生了显著变化,单一针叶处理与针阔混合处理间18S rDNA基因泳道带型的相似度只有28%,并且单一马尾松处理土壤18S rDNA的丰富度和多样性显著高于马尾松+白栎和马尾松+青冈,单一湿地松处理土壤18S rDNA的丰富度也显著高于湿地松+白栎和湿地松+青冈,多样性显著高于湿地松+白栎处理;3)土壤真菌18S rDNA的丰富度与凋落物初始C含量呈显著正相关,与凋落物初始N含量呈显著负相关.针叶凋落物中引入阔叶凋落物后,增加了凋落物中N的比重,C的比重则下降,显著影响了土壤真菌群落的结构.
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| 关 键 词： | 森林凋落物 混合分解 土壤微生物 群落结构 16S rDNA 18S rDNA 多样性 DGGE |
Analyzing the Effect of Mixed Decomposition of Conifer and Broadleaf Litters on Soil Microbial Communities by Using PCR-DGGE |
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| CHEN Falin,ZHANG Kai,ZHENG Hua,LIN Xueqiang,OUYANG Zhiyun & TU Naimei.Analyzing the Effect of Mixed Decomposition of Conifer and Broadleaf Litters on Soil Microbial Communities by Using PCR-DGGE[J].Chinese Journal of Applied and Environmental Biology,2011(2):145-151. |
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| Authors: | CHEN Falin ZHANG Kai ZHENG Hua LIN Xueqiang OUYANG Zhiyun & TU Naimei |
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| Institution: | CHEN Falin1,ZHANG Kai1,ZHENG Hua1,LIN Xueqiang2,OUYANG Zhiyun1 & TU Naimei3(1State Key Laboratory of Urban and Regional Ecology,Research Center for Eco-Environmental Sciences,Chinese Academy of Sciences,Beijing 100085,China)(2Hainan Research Academy of Environmental Sciences,Haikou 570206,China)(3College of Agronomy,Hunan Agricultural University,Changsha 410128,China) |
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| Abstract: | Clarifying the microbiological mechanism of litter decomposition is of great significance,especially in understanding how the soil microbial communities affect the decomposition of mixed litters with conifer and broadleaf trees.In this study,a simulation experiment was designed with flowerpots,and the litters of the typical species in the southern hilly red soil region,including Pinus massoniana(PM),Pinus elliottii(PE),Quercus fabri(QF) and Cyclobalanopsis glauca(CG),were investigated as study materials.The... |
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| Keywords: | forest litter decomposition of mixed litter soil microbe community structure 16S rDNA 18S rDNA diversity DGGE |
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