| 响应面法优化锰氧化细菌发酵培养基 |
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| 引用本文： | 刘乐乐,鲍蓬,廖蓓,苏键镁,王阶平,何进.响应面法优化锰氧化细菌发酵培养基[J].应用与环境生物学报,2011(1):130-134. |
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| 作者姓名： | 刘乐乐 鲍蓬 廖蓓 苏键镁 王阶平 何进 |
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| 作者单位： | 华中农业大学农业微生物学国家重点实验室/微生物农药国家工程研究中心; |
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| 基金项目： | 国家自然科学基金项目(No.40830527)资助~~ |
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| 摘 要： | Bacillus sp.WH4是一株锰氧化能力强的锰氧化细菌,在锰污染的水源净化方面具有较好的应用前景.为提高发酵液中Bacillus sp.WH4的活菌数,应用响应面法优化其发酵培养基.Plackett-Burman设计获得对活菌数有显著影响的4个因素:豆粕粉、KH2PO4、MgSO4、FeSO4;最陡爬坡试验确定中心组合实验的最大响应区域;中心组合设计和响应面分析优化出4个显著因素的最佳浓度.优化后的最适培养基(w/%)组成为:蔗糖2、豆粕粉0.96、K2HPO4 0.8、KH2PO4 0.19、MgSO4.7H2O 0.11、CaCl2 0.02、NaCl 0.5、FeSO4.7H2O 0.02.发酵试验表明,最优培养基的活菌数可达4.2×109 CFU mL-1,比初始发酵培养基提高近3倍.
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| 关 键 词： | 锰氧化细菌 生物除锰 培养基优化 响应面法 Plackett-Burman设计 |
Optimization of Fermentation Medium for A Manganese-oxidizing Bacterium Bacillus sp. WH4 by Response Surface Method |
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| LIU Lele,BAO Peng,LIAO Bei,SU Jianmei,WANG Jieping & HE Jin.Optimization of Fermentation Medium for A Manganese-oxidizing Bacterium Bacillus sp. WH4 by Response Surface Method[J].Chinese Journal of Applied and Environmental Biology,2011(1):130-134. |
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| Authors: | LIU Lele BAO Peng LIAO Bei SU Jianmei WANG Jieping & HE Jin |
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| Institution: | LIU Lele,BAO Peng,LIAO Bei,SU Jianmei,WANG Jieping & HE Jin(State Key Laboratory of Agricultural Microbiology and National Engineering Research Center of Microbial Pesticides,Huazhong Agricultural University,Wuhan 430070,China) |
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| Abstract: | Bacillus sp.WH4 is a manganese-oxidizing bacterium possessing the strong activity to oxidize soluble manganese ion into insoluble manganese dioxide.It would be a promising application candidate for the cleaning of water resources polluted by manganese.To maximize the viable cell of Bacillus sp.WH4 in a fermentation broth,its fermentation medium was optimized using response surface method(RSM).Firstly,four significant factors,soybean meal,KH2PO4,MgSO4 and FeSO4,were screened out by Plackett-Burman(PB) design... |
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| Keywords: | manganese-oxidizing bacteria biological manganese removal medium optimization response surface method Plackett-Burman design |
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