| Bt肠毒素基因与plcR基因的相关性及肠毒素对小鼠的急性毒性 |
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| 引用本文： | 黄必旺,林晓东,苏芙蓉,关雄. Bt肠毒素基因与plcR基因的相关性及肠毒素对小鼠的急性毒性[J]. 应用与环境生物学报, 2011, 0(3): 418-421 |
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| 作者姓名： | 黄必旺 林晓东 苏芙蓉 关雄 |
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| 作者单位： | 福建农林大学生物农药与化学生物学教育部重点实验室; |
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| 基金项目： | 国家“863”计划项目(No.2006AA10A212); 科技部农业科技成果转化资金(No.2010GB2C400212); 福建省自然科学基金项目(No.2010J01069); 福建省高校服务海西建设重点项目(No.0b08b005)资助~~ |
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| 摘 要： | 用PCR方法检测溶血肠毒素BL、肠毒素T和肠毒素S三种肠毒素基因(hblA、becT、entS)及毒素调控基因plcR在30株苏云金芽胞杆菌株(Bt)中的分布.结果表明,含有hblA基因、entS基因、becT基因及plcR基因片段的Bt菌株分别占66.7%、70%、70%和73.3%.其中,含有plcR基因的菌株都至少含有一种肠毒素基因,不含肠毒素基因的菌株也未检测到plcR基因,说明plcR基因与肠毒素基因有着密切的关系.用3个Bt菌株WB9、HD2和HD9(都含有hblA、becT、entS和plcR基因片段,HD2和HD9经RPLA与TECRA肠毒素检测试剂盒检测具有高滴度)对小白鼠进行口服急性毒性试验.结果表明,所有3种供试菌株的发酵上清液对小白鼠行为和健康没有明显影响,对内部器官(心、肝、肺等)也没有产生病理现象.图5表3参14
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| 关 键 词： | 苏云金芽胞杆菌 肠毒素基因 调控基因 急性毒性 |
Correlation of Enterotoxin Gene and plcR Gene in Bacillus thuringiensis and Toxicity of Enterotoxin to Mice |
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| HUANG Biwang,LIN Xiaodong,SU Furong , GUAN Xiong. Correlation of Enterotoxin Gene and plcR Gene in Bacillus thuringiensis and Toxicity of Enterotoxin to Mice[J]. Chinese Journal of Applied and Environmental Biology, 2011, 0(3): 418-421 |
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| Authors: | HUANG Biwang LIN Xiaodong SU Furong & GUAN Xiong |
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| Affiliation: | HUANG Biwang,LIN Xiaodong,SU Furong & GUAN Xiong(Key Laboratory of Ministry of Education for Biopesticide and Chemical Biology,Fujian Agriculture and Forestry University,Fuzhou 350002,China) |
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| Abstract: | The presence of hemolysin Hb1A gene(hblA),enterotoxin T gene(bceT),enterotoxin S gene(entS) and toxin regulatory gene plcR in 30 Bacillus thuringiensis strains was analyzed by PCR.The results showed that hb1A gene sequence could be detected in 20 strains(66.7%),both bceT and entS gene sequences could be detected in 21 strains(70%),and plcR gene sequence in 22 strains(73.3%).The strains that included plcR gene sequence also contained at least one enterotoxin gene sequence,and those that lacked enterotoxin ge... |
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| Keywords: | Bacillus thuringiensis enterotoxin gene regulatory gene toxicity |
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