| 蛋白酶对水环境中病毒的灭活作用 |
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| 引用本文： | 吕文洲,杨清香,张昱,杨敏,朱春芳.蛋白酶对水环境中病毒的灭活作用[J].环境科学,2004,25(5):93-96. |
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| 作者姓名： | 吕文洲 杨清香 张昱 杨敏 朱春芳 |
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| 作者单位： | 中国科学院生态环境研究中心环境水质学国家重点实验室,北京,100085;宁波大学建筑工程与环境学院,浙江宁波,315211;中国科学院生态环境研究中心环境水质学国家重点实验室,北京,100085;中国地质大学水资源与环境工程学院,北京,100083 |
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| 基金项目： | 国家自然科学基金主任基金项目(20347001) |
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| 摘 要： | 以大肠杆菌噬菌体T4作为模式病毒,研究了蛋白酶对病毒的灭活作用.试验结果表明:蛋白酶灭活病毒的效果明显. 适宜条件下,67.5u/mL的蛋白酶K处理1h对纯水中病毒(6.2×105PFU/L)和生活污水中病毒(7.2×105PFU/L)灭活率分别达到了99.4%和49.4%,处理3h的灭活率分别是>99.9%和81.1%;工业蛋白酶1398在75.0u/mL酶浓度下处理1h对纯水水中病毒(1.7×105 PFU/L)灭活率达到74.4%.pH和温度对病毒灭活效果的影响不明显.
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| 关 键 词： | 蛋白酶 病毒 噬菌体T4 灭活 消毒 |
| 文章编号： | 0250-3301(2004)05-0093-04 |
| 收稿时间： | 2003/10/31 0:00:00 |
| 修稿时间： | 2003/12/23 0:00:00 |
Inactivation of T4 Phage in Water Environment Using Proteinase |
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| Lü Wen-zhou,Yang Qing-xiang,Zhang Yu,Yang Min,Zhu Chun-fang.Inactivation of T4 Phage in Water Environment Using Proteinase[J].Chinese Journal of Environmental Science,2004,25(5):93-96. |
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| Authors: | Lü Wen-zhou Yang Qing-xiang Zhang Yu Yang Min Zhu Chun-fang |
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| Institution: | SKLEAC, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China. |
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| Abstract: | The inactivation effectiveness of proteinase to viruses was investigated by using T4 phage as a model virus. The results showed that the inactivation effectiveness of proteinase to T4 phage was obvious. In the optimum conditions and 67.5u/mL concentration, the inactivation rate of proteinase K to T4 phage in sterilized water and in sewage achieved 99.4% and 49.4% respectively in an hour, and achieved >99.9% and 81.1% in three hours. The inactivation rate of the industrial proteinase 1398 to T4 phage in sterilized water achieved 74.4% in an hour. The effects of pH and temperature on the inactivation effectiveness was not evident. |
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| Keywords: | proteinase virus inactivation T4 phage disinfection |
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