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| 自然土壤中苯酚降解菌的分离和系统发育分析 | |
| 引用本文： | 王云端,董小军,王茜,洪青,蒋新,李顺鹏. 自然土壤中苯酚降解菌的分离和系统发育分析[J]. 环境科学, 2007, 28(3): 623-626 |
| 作者姓名： | 王云端 董小军 王茜 洪青 蒋新 李顺鹏 |
| 作者单位： | 中国科学院南京土壤研究所土壤与农业可持续发展国家重点实验室,南京,210008;南京农业大学生命科学学院,农业部农业环境微生物工程重点开放实验室,南京,210095;南京农业大学生命科学学院,农业部农业环境微生物工程重点开放实验室,南京,210095;中国科学院南京土壤研究所,土壤与农业可持续发展国家重点实验室,南京,210008 |
| 基金项目： | 国家自然科学基金项目(30400013,40471073);江苏省科技厅项目(BG2005322);土壤与农业可持续发展国家重点实验室开放基金项目(055112);南京农业大学SRT项目(0506A10) |
| 摘 要： | 采用富集培养的方法,从未受苯酚污染的自然土壤中分离得到5株能以苯酚为唯一碳源生长的细菌.根据生理生化特性和系统发育分析对它们进行了初步鉴定,菌株PHD-2为Ralstonia sp.,菌株 PHD-4为Acinetobacter sp.,菌株PHD-5 为Microbacterium sp.,菌株PHD-1和PHD-3均为Pseudomonas sp.,16S rRNA基因序列的同源性比较和系统发育分析表明自然土壤中的苯酚降解菌具有丰富的多样性. |
| 关 键 词： | 自然土壤 苯酚降解菌 系统发育分析 |
| 文章编号： | 0250-3301（2007）03-0623-04 |
| 收稿时间： | 2006-04-29 |
| 修稿时间： | 2006-04-292006-06-23 |
Isolation of Phenol-Degrading Bacteria from Natural Soil and Their Phylogenetic Analysis |
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| WANG Yun-duan,DONG Xiao-Jun,WANG Xi,HONG Qing,JIANG Xin and LI Shun-peng. Isolation of Phenol-Degrading Bacteria from Natural Soil and Their Phylogenetic Analysis[J]. Chinese Journal of Environmental Science, 2007, 28(3): 623-626 | |
| Authors: | WANG Yun-duan DONG Xiao-Jun WANG Xi HONG Qing JIANG Xin LI Shun-peng |
| Affiliation: | 1. State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008, China; 2. Key Laboratory of Microbiological Engineering Agricultural Environment, Ministry of Agriculture, College of Life Science, Nanjing Agricultural University, Nanjing 210095, China |
| Abstract: | Five bacterial strains capable of utilizing phenol as sole carbon source for growth were isolated from non-contaminated natural soil sample after enrichment in the presence of phenol. They were preliminarily identified according to their phylogenetic analysis and physiological and biochemical characteristics. Strain PHD-2, PHD-4 and PHD-5 belonged to the genera of Ralstonia, Acinetobacter and Microbacterium respectively; strain PHD-1 and PHD-3 were from the genus of Pseudomonas. Homology comparing of their 16S rRNA gene sequences and phylogenetic analysis displayed the high biodiversity of phenol-degrading microorganisms in the natural soil. |
| Keywords: | natural soil phenol-degrading microorganisms phylogenetic analysis |
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