Detection of Escherichia coli in wastewater based on enzyme immunoassay

This research describes a fast detection method on the basis of enzyme-linked immunosorbent assay (ELISA) for Escherichia coli in drainage of wastewater treatment plants. Optimized conditions such as the reaction format (sandwich or direct), the concentrations of diluted horseradish peroxidase (HRP)-E. coli conjugate, and anti-HPR antibody and pretreatment of E. coli were studied. Those results showed that the linear range of detection for E. coli was 10 cfu/mL-6 × 10⁴ cfu/mL. Compared with conventional methods, it is a convenient and sensitive detection method with low cost. Translated from Environmental Science, 2005, 26(5): 128–131 译自: 环境科学]