绿色荧光蛋白标记阿特拉津降解基因工程菌的特性

通过转化绿色荧光蛋白基因质粒,对阿特拉津降解基因工程菌进行标记.转化后,绿色荧光蛋白在细胞内表达情况良好.在含抗生素的LB培养基中,绿色荧光蛋白的表达水平高于LB培养基和基础培养基.在细胞生长的稳定期,绿色荧光蛋白表达水平高于停滞期和对数生长期.绿色荧光蛋白基因质粒转化和表达,不会对基因工程菌原有的降解能力产生影响,而且绿色荧光蛋白的表达水平和降解活性存在接近线形的正相关关系.荧光蛋白标记细胞投加到反应器活性污泥中,会以2种状态存在:游离态和附着态,而且初期游离态细胞的数量大于附着态细胞的数量.

Characteristics of Atrazine-Degrading Genetically Engineered Microorganism(GEM) Labeled with Green Fluorescent Protein(GFP)

The atrazine-degrading genetically engineered microorganism(GEM) was labeled by transforming a plasmid containing green fluorescent protein(GFP) gene.GFP gene could be expressed well in the cell of GEM after transforming.In the LB medium containing antibiotic,the expressing level of GFP was higher than in pure LB medium and in the basic medium.In the stable phase of cell growth,the expressing level of GFP was higher than in the lag phase and in the logarithmic phase.The transformation and expression of GFP gene wouldn't affect the degrading ability of GEM.The level of GFP expression was almost linear with the degrading activity.There exist two states of cells after inoculating GEM labeled with GFP into activated sludge: suspended state and adhered state to sludge floc,and the amount of suspended cells is higher than that adhered to sludge floc.