电氧化杀藻过程中藻细胞活性变化的研究

由Ti/RuO2棒为阳极、不锈钢管为阴极组成电氧化管式反应装置,采用TTC-脱氢酶活性和中性红染色的活性测定方法,研究了电氧化杀藻中藻活性变化以及影响因素.结果显示,在8mA/cm2下处理30min后,以藻细胞密度计的杀藻率仅为45%,而此时TTC-脱氢酶活性下降了94%,中性红染色率为100%,藻细胞已无法再生长,已全部失活,表明活性指标比细胞密度更能准确反映杀藻处理效果.电解液种类对杀藻存在明显影响,常用电解液Na2SO4和NaNO3的杀藻效果相近,Na2SO4浓度对杀藻效果影响不大.但当电解液中含有0.1mmol/LNaCl时,杀藻效果明显提高,5min后的脱氢酶下降了87%,中性红染色率达到82%.藻样初始浓度对杀藻效果影响较大,细胞密度增大,杀藻效果急剧下降,以天然水为电解液,细胞密度达到水华时的4.4×107个/L,只需处理1min可将藻细胞全部灭活.

Study on the Variation of Algal Activity During the Electrochemical Oxidation as Inactivation Method

The paper studied the variation of algal activity during the electrochemical inactivation and the influence factors by the use of TTC-dehydrogenase activity and neutral red staining assays. The treatment reactor was consisted of Ti/RuO₂ rod as anode and stainless steel pipe as cathode. The results showed that algal inactivation rate was 45% in cell density after 30 min treatment at 8 mA/cm². Whereas the decrease of TTC-dehydrogenase activity was 94% and neutral red staining percentage was 100%. The algae after treatment was unable to regrow and it revealed that the algal activity assays can reflect the inactivation effect more correctly than cell density. The electrolytes could influence the inactivation efficiency. The electrolytes of Na₂SO₄ and NaNO₃ had similar effects on algal inactivation and Na₂SO₄ concentration had small influence on the treatment. However, when the electrolyte contained 0.1 mmol/L NaCl, the algal inactivation was improved obviously with the 87% for TTC-dehydrogenase activity decrease and 82% for neutral red staining ratio. The initial algal concentration also influenced the treatment efficiency. If cell density increased, the inactivation efficiency decreased significantly. All algal cells in samples with cell density of 4.4×10⁷ cells/L were completely inactivated by the use of natural water as electrolyte within 1 minute.