Genotoxic effects of Bismuth (III) oxide nanoparticles by Allium and Comet assay |
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| Institution: | 1. School for Radiological and Interdisciplinary Sciences (RAD-X) and Collaborative Innovation Center of Radiation Medicine of Jiangsu Higher Education Institutions, Soochow University, Suzhou, Jiangsu Province, 215123, China;2. The Second Affiliated Hospital of Soochow University, 1055 Sanxiang Road, Suzhou, Jiangsu Province, 215004, China;3. Department of Toxicology, School of Public Health, Soochow University, Suzhou, Jiangsu Province, 215123, China;4. Institute of Chinese MateriaMedica, Shanghai University of Traditional Chinese Medicine, Shanghai, 201203, China |
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| Abstract: | Genotoxic effects of Bismuth (III) oxide nanoparticles (BONPs) were investigated on the root cells of Allium cepa by Allium and Comet assay. A. cepa roots were treated with the aqueous dispersions of BONPs at five different concentrations (12.5, 25, 50, 75, and 100 ppm) for 4 h. Exposure of BONPs significantly increased mitotic index (MI) except 12.5 ppm, total chromosomal aberrations (CAs) in Allium test. While stickiness chromosome laggards, disturbed anaphase–telophase and anaphase bridges were observed in anaphase–telophase cells, pro-metaphase and c-metaphase in other cells. A significant increase in DNA damage was also observed at all concentrations of BONPs except 12.5 ppm by Comet assay. The results were also analyzed statistically by using SPSS for Windows; Duncan’s multiple range test was performed. These results indicate that BONPs exhibit genotoxic activity in A. cepa root meristematic cells. |
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| Keywords: | Allium Chromosome aberration Comet DNA damage |
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