| SDS－PAGE和HPLC－荧光检测分离鉴定恩施高硒区大豆中含硒蛋白 |
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| 引用本文： | 谢申猛,王子健,彭安. SDS－PAGE和HPLC－荧光检测分离鉴定恩施高硒区大豆中含硒蛋白[J]. 环境科学, 1994, 0(5) |
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| 作者姓名： | 谢申猛 王子健 彭安 |
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| 作者单位： | 中国科学院生态环境研究中心 |
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| 摘 要： | 采用凝胶电泳技术分离恩施高硒地区大豆中的含硒蛋白组分，分辨出２７条蛋白条带。用高效液相色谱－荧光测定法对这些条带分别进行硒含量测定并进行空白扣除，发现其中有１３条带含硒。用标准分子量蛋白标定含硒蛋白组分分子量分别为：５８．１－６０．３；５２．５－５３．７；４６．８－５０．１；２９．５－３０．９；２８．８；２５．１－２５．７；２４．３；１９．７－２０．９；１８．４－１８．６；１６．８－１７．９；１６．１－１６．２；１５．２－１５．８和１４．３－１４．８ｋＤａ。
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| 关 键 词： | 凝胶电泳，高效液相色谱，荧光检测，含硒蛋白 |
SDS-PAGE Seperation and HPLC- FID Identification of Selenoproteins in Soybean from Enshi Area Having a Higher Level of Selenium in Soil |
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| Xie Shenmeng,Wang Zijian and Peng An. SDS-PAGE Seperation and HPLC- FID Identification of Selenoproteins in Soybean from Enshi Area Having a Higher Level of Selenium in Soil[J]. Chinese Journal of Environmental Science, 1994, 0(5) |
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| Authors: | Xie Shenmeng Wang Zijian Peng An |
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| Abstract: | Selenoproteins in soybean from Ensh area where selenium in soil was at a higher level were seperated by using sodium dodecyl sulfste-polyacrylamide gel electrophoresis (SDS-PAGE) and then identified by using high Performance liquid chromatography with a fluorescent indication detector (HPLC-FID). Of 27 protein or protein subunit bands identified, 13were found to be selenium species. According to a standard protein kit, their molecular weights were estimated at 58. 3- 60. 3, 52. 5- 53. 7, 46.8- 50. 1,29. 5-30. 9, 28. 8, 25. 1- 25. 7, 24. 3,19. 7-20. 9,18. 4-18. 6, 16. 8- 17. 9, 16. 1-16.2, 15. 2-15. 8 and 14. 3- 14. 8 KDa,respectively. |
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| Keywords: | SDS-PAGE HPLC-FID selenoprotein speciation. |
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