| 饮用水中几种细菌计数方法的比较 |
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| 引用本文： | 鲁巍,王云,张晓健.饮用水中几种细菌计数方法的比较[J].环境科学,2004,25(4):167-169. |
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| 作者姓名： | 鲁巍 王云 张晓健 |
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| 作者单位： | 清华大学环境科学与工程系,北京,100084;清华大学环境科学与工程系,北京,100084;清华大学环境科学与工程系,北京,100084 |
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| 基金项目： | 国家自然科学基金资助项目(50238020);国家高技术研究发展计划(863计划)资助项目(2002AA601140) |
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| 摘 要： | 比较采用不同培养基的平板计数(Plate Counts,PC)方法,以及不同荧光染色剂的显微镜直接计数方法与常规计数方法的差别.研究认为,常规平板计数方法并不能准确反映饮用水中实际存活的细菌数量;吖啶橙直接计数(Acridine Orange Direct Counts,AODC)的结果最高,较常规平板计数方法高出3～4个量级;活细菌直接计数(Direct Viable Counts,DVC)中,DVC-N.A.、DVC-CTC和DVC-BacLight等计数方法的结果较常规平板计数结果高出2～3个量级.以地表水为水源的水厂出水中活细菌数占总细菌数的比例在10%左右.
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| 关 键 词： | 饮用水 直接计数 平板计数 活细菌直接计数 细菌 |
| 文章编号： | 0250-3301(2004)04-0167-03 |
| 收稿时间： | 8/7/2003 12:00:00 AM |
| 修稿时间： | 2003/9/25 0:00:00 |
Methods of Enumeration of Bacteria in Drinking Water |
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| LU Wei,WANG Yun and ZHANG Xiao-jian.Methods of Enumeration of Bacteria in Drinking Water[J].Chinese Journal of Environmental Science,2004,25(4):167-169. |
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| Authors: | LU Wei WANG Yun and ZHANG Xiao-jian |
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| Institution: | Department of Environmental Science and Engineering, Tsinghua University, Beijing 100084, China. |
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| Abstract: | Methods of the enumeration of total bacteria and Coliform in drinking water were researched in this paper. The differences between heterotrophic plate count and direct viable count method were compared. It is concluded that the total number of bacteria in R2A medium is one order of quantity higher than the traditional plate count agar, and the results of acridine orange direct counts(AODC) is the highest. Some different staining methods in direct viable count were also compared in this paper, such as nalidixic acid, CTC staining and BacLight staining. The proportion of the live bacteria to the dead is about 10%. |
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| Keywords: | drinking water enumeration PC DVC bacteria |
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