高效纤维素分解菌复合系的构建及其环境适应性研究

以纤维素培养基和纤维素刚果红培养基为初筛和复筛培养基，从采集的样品中分离得到具有纤维素分解能力的菌株20株。选择其中具有较高纤维素酶活力的12个菌株与2株自生固氮菌和EM菌进行正交实验，得到3个组合表现出协同降解纤维素的作用，对滤纸降解效果为组合12〉组合8〉组合5。选用组合12进行了一系列的环境适应性研究，结果表明，碳源为微晶纤维素＋CMC-Na时CMC酶活最高，最适氮源为蛋白胨＋酵母膏，最适pH6．5，最适培养温度为30℃。培养第6天CMC酶活最高。

Construction of a Complex Microbial System with Efficient Cellulose Decomposition Bacteria and Its Environmental Adaptability

Cellulose and Congo red agar were used as medium for initial isolation and re-isolation of cellulose decomposition bacteria. From samples collected, 20 strains of bacteria showing ability of cellulose decomposition were isolated. 12 strains of the isolated 20 strains were selected to conduct an orthogonal test with 2 strains of asotohacter and Effective Microorganisms. Three groups of bacteria with synexgy for cellulose decomposition were obtained. The degradation rate for filter paper of the groups showed that Group 12 〉 Group 8 〉 Group 5. The Group 12 was used to study its environmental adaptability. It was showed that the optimal conditions as follows： when microcrystalline cellulose and CMC-Na were used as carbon source, CMCase reaches the maximum ; the optimal nitrogen source was peptone and yeast extract; the optimal pH was 6. 5 ; the optimal culture temperature was 30℃ and CMCase reached its maximcm at the sixth day, respectively.