燃煤细颗粒物对血管内皮细胞EA.hy926的细胞毒性

以银川散煤为样品煤,在实验室采用固定源稀释通道采集燃煤PM2.5,超声波水浴提取燃煤PM2.5全颗粒悬液,对人脐静脉内皮细胞EA.hy926进行染毒,并采用MTS法检测燃煤PM2.5在不同染毒时间对EA.hy926细胞增殖的影响.结果表明,燃煤PM2.5悬液对EA.hy926细胞分别染毒6,12,24h均可抑制细胞增殖,且12,24h各剂量组和溶剂对照相比具有统计学意义;在相同染毒剂量组内,和6,12h相比,染毒24h对细胞存活率抑制更加明显,其差异具有统计学意义.可见,燃煤PM2.5可以抑制血管内皮细胞增殖且具有时间和剂量依赖性,血管内皮损伤是PM2.5致心血管毒性的可能机制之一.

Cytotoxicity assessment of fine particles from coal combustion on EA.hy926 vascular endothelial cells.

The coal sample taken from Yinchuan,coal-fired PM2.5 was sampled by fixed source dilution channel and PM2.5 suspension was extracted by ultrasonic water-bath method.Human umbilical vein endothelial cells were treated with PM2.5 suspension at various concentrations and exposed for different times(6h,12h,and 24h).The MTS assay was used to measure the effect of PM2.5 on cell proliferation of EA.hy926.Coal-fired PM2.5 suspension decreased the viability of vascular endothelial cells at different times and exposure time groups of 12h and 24h showed significance statistically in comparison to the solvent control.Compared with the same concentration group of 12h and 6h,exposure time groups of 24h had statistically significant differences.Coal-fired PM2.5 could significantly decrease the viability of hman umbilical vein endothelial cells in dose-dependent and time-dependent manner.Vascular endothelial injury might be one of the possible mechanisms of cardiovascular toxicity caused by coal-fired PM2.5.