低浓度Pb2+、Cd2+对鲫鱼肝脏组织中 HSP70诱导的影响

以鲫鱼(Carassius auratus)作为实验对象,经过40 d Pb2+、Cd2+不同浓度的暴露后,运用SDS-PAGE和Western Blotting方法检测鱼肝脏组织内应激蛋白HSP70的诱导表达情况.结果表明,在实验浓度下,与对照组相比,Pb2+、Cd2+对鱼肝脏内HSP70有显著的诱导(P＜0.05),但在Cd2+浓度为0.2 mg/L有停止表达HSP70的现象,可能是由于浓度过高造成了组织病理损伤而破坏了诱导表达机制.实验还发现,在实验浓度低于国家渔业用水标准时,HSP70仍然表现为明显诱导(P＜0.05),充分说明运用分子生物学指标要比传统的环境检测指标敏感,具有对污染物早期预警的作用.

Effects of Pb2+ and Cd2+ on the induction of HSP70 in the fish liver

The effects of lead (Pb+{2+}) and cadmium (Cd+{2+}) on the induction of HSP70 in the fish liver were studied .After the fish %(Carassius auratus)% were exposed at the experimental concentration for forty days,the SDS PAGE and Western Blotting technique were used to detect the HSP70 in the fish liver. That the test groups exposed to the lead (Pb+{2+}) and cadmium (Cd+{2+}) all showed a significant increase in HSP70 as a function of exposure (%P%<0.05),but at the concentration of 0.1 mg/L ,it was found that the induction of HSP70 has been stopped perhaps for the high concentration of the toxic heavy metal cadmium (Cd+{2+}). It was also found that the fish exposed to the concentration of Pb+{2+} and Cd+{2+} which below the national fishery water quality standard also have a significant increase in HSP70. It means that the low concentration of the heavy metal also can harm the fish body. So HSP70 as a environmental biomarker can be a more sensitive and rapid indicator than conventional end points such as the growth death.