Effects of tri-o-cresyl phosphate on serum estrogen and progesterone concentration and ATPase activity in the shell gland of adult hens

The activities of calcium-activated ATPase (Ca2+-ATPase) and calcium magnesium-activated ATPase (Ca2+-Mg2+-ATPase) in the shell gland, and concentrations of 17beta-estradiol (E2) and progesterone in serum were monitored, respectively, from hens orally dosed with tri-o-cresyl phosphate (TOCP) (750 mg/kg). Treated birds were monitored daily for laying and development of delayed neurotoxicity, and activities of Ca2+-ATPase and Ca2+-Mg2+-ATPase were measured at 7 and 10 days after dosing. TOCP-treated birds manifested motor deficit by 7-9 days postdosing, while hens administered vehicle exhibited no signs of delayed neurotoxicity. Ca2+-ATPase and Ca2+-Mg2+-ATPase activities of shell glands from TOCP-dosed hens were not significantly affected (P > 0.05). The serum E2 concentration was significantly reduced in TOCP-treated hens (P < 0.01); however, progesterone levels were unaffected.