常规液相色谱柱测定早熟素Ⅱ的方法探讨

通过使用市售的常规C18液相色谱柱(规格:150mm×4.6mm,5 μm),探讨了早熟素Ⅱ的分析方法并对其进行了可行性论证.以V(甲醇):V(水)=80:20为流动相,流量1 mL·min~(-1),检测波长为224nm,柱温35℃.得出早熟素Ⅱ在8～108μg·mL~(-1)的范围内呈良好的线性关系(相关系数,为0.99997),最低检出限为1.0379μg·mL~(-1)(信噪比为3),加标回收率为93.3%～104.98%,变异系数为0.12%～4.41%.与使用非常规色谱柱分析早熟索Ⅱ的方法相比,该方法采用了市售常规柱,能够更大限度地满足实验条件,通用性更强.

Method for determination of precocene Ⅱ with conventional liquid chromatographic column

A method for determination of precocene Ⅱ was developed by HPLC with conventional C18 column(150 mm×4.6 mm, 5 um)and its feasibility assessment was made. The samples were analyzed on a Luna C18 column using methyl alcohol- ultrapure water (80 : 20, V/V) as the mobile phase at a flow rate of 1 mL·min~(-1). The detection wavelength was 224 nm and the column tem-perature was 35 ℃. Well linear relationship was showed at the range of 8～108 μg·mL~(-1) and correlation coefficient of the calibration curve was 0.999 97. The detection limit was 1.037 9 μg·mL~(-1) (S/N=3). The recovery of added sample ranged from 93.3% to 104.98% with the relative standard deviations of 0.12%～4.41%. Comparing with other measuring methods using unconventional column, better versatility was manifested by the method in which a conventional liquid chromatographic column which was easily available in the market was employed and expetimental conditions were met to a great extent.