| 微囊藻毒素的提取和提纯研究 |
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| 引用本文： | 闫海,潘纲,张明明,陈海柳,邹华.微囊藻毒素的提取和提纯研究[J].环境科学学报,2004,24(2):355-359. |
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| 作者姓名： | 闫海 潘纲 张明明 陈海柳 邹华 |
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| 作者单位： | 中国科学院生态环境研究中心,环境水质学国家重点实验室,北京,100085;中国科学院生态环境研究中心,环境水质学国家重点实验室,北京,100085;中国科学院生态环境研究中心,环境水质学国家重点实验室,北京,100085;中国科学院生态环境研究中心,环境水质学国家重点实验室,北京,100085;中国科学院生态环境研究中心,环境水质学国家重点实验室,北京,100085 |
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| 基金项目： | 国家自然科学基金 (编号 :30 2 70 2 77,2 0 3770 4 7和 2 0 1770 2 9)，973项目 (2 0 0 2CB4 12 30 8)，86 3项目 (2 0 0 2AA6 0 112 0 ) |
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| 摘 要： | 对采自云南滇池水华蓝藻细胞中的微囊藻毒素 (Microcystins,MCs)的提取与提纯方法进行了研究 .结果表明 ,在藻细胞干重浓度为 2 0g·L-1下 ,与不同甲醇浓度提取液相比 ,4 0 %甲醇溶液可以最有效地从藻细胞中提取出MC RR和MC LR .将MCs提取液过Waters固相萃取小柱后 ,用 70 %甲醇溶液洗脱吸附于柱上的MCs ,可以分别获得 7 3%和 3 5 %纯度的MC RR和MC LR .通过观察洗脱液的颜色变化 ,收集蓝绿色和橘黄色后面流出的基本无色的洗脱液 ,可以获得纯度为 2 8 6 %和12 9%的MC RR和MC LR .
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| 关 键 词： | 微囊藻毒素 提取 提纯 |
| 文章编号： | 0253-2468(2004)02-0355-05 |
| 收稿时间： | 2003/4/11 0:00:00 |
| 修稿时间： | 2003年4月11日 |
Study on the extraction and purification of microcystins |
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| YAN Hai,PAN Gang,ZHANG Mingming,CHEN Hailiu and ZOU Hua.Study on the extraction and purification of microcystins[J].Acta Scientiae Circumstantiae,2004,24(2):355-359. |
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| Authors: | YAN Hai PAN Gang ZHANG Mingming CHEN Hailiu and ZOU Hua |
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| Institution: | State Key Laboratory of Environmental Aquatic Chemistry, Research Center for Eco-environmental Sciences, Chinese Academy of Sciences, Beijing 100085,State Key Laboratory of Environmental Aquatic Chemistry, Research Center for Eco-environmental Sciences, Chinese Academy of Sciences, Beijing 100085,State Key Laboratory of Environmental Aquatic Chemistry, Research Center for Eco-environmental Sciences, Chinese Academy of Sciences, Beijing 100085,State Key Laboratory of Environmental Aquatic Chemistry, Research Center for Eco-environmental Sciences, Chinese Academy of Sciences, Beijing 100085 and State Key Laboratory of Environmental Aquatic Chemistry, Research Center for Eco-environmental Sciences, Chinese Academy of Sciences, Beijing 100085 |
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| Abstract: | The methods for extracting and purifying microcystin (MCs) in dry cyanobacterial cells taken from Dianchi Lake, Yunnan Province, were studied in this paper. 40% methanol-water solution was found to be an optimum solution for effectively extracting MC-RR and MC-LR from cyanobacterial cells among different ratios of methanol when the concentration of dry cyanobacterial cells was 20g·L-1. After the extraction solution of 40% methanol containing MCs was passed through a cartridge (Waters, OASIS~(TM) HLB Cartridge), MCs were purified by the elution with 70% methanol, and 7.3% and 3.5% purities of MC-RR and MC-LR were obtained. By collecting the achromatic fraction of the elution after blue-green and orange-yellow color elution, MC-RR and MC-LR with the purities of 28.6% and 12.9% were obtained. |
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| Keywords: | microcystins extraction purification |
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