微生物絮凝剂A3的培养条件及处理啤酒废水条件研究

从土壤中筛选出一株产微生物絮凝剂的菌株A3,利用均匀设计安排试验,用曲线拟和方法创建数学模型的方法研究了其产絮凝培养基条件,结果表明:葡萄糖添加量2.2%、酵母膏添加量0.51%、pH7.6、温度30.3℃时A3产絮凝剂条件最佳,数学模型为Y=-486.2+60.5X1-13.7X12+61.9X2-60.7X22+56X3-3.7X32+18.2X4-0.3X42,培养的动力学模型为:(C0-C)t/C0×100%=96.03-338.15/t。同时利用A3菌产絮凝剂对啤酒废水进行COD去除处理,结果显示:pH值为8、100mL废水中絮凝剂的添加量为1.5mL和1%CaCl2用量为2mL时,COD去除效果最佳。在最佳工艺条件下的COD去除率可达60%。并构建了相应的数学模型为:y=40.8+22.4e-(x-8)2/4.5;y=66.4-58.9/(1+(x/0.67)6.5)和y=67.4-56.5/(1+(x/0.68)4.86)。

Cultivation of Microbial Flocculants and Optimization of Treatment Condition for Brewery Wastewater

One strain of microbial flocculant-producing bacterial A3 was screened from soil,the culture medium of microbial flocculant-producing was optimized through application of homogeneous design arranging experiment and establishment of mathematical model by curve fit method.Results indicated that the best conditions of flocculant-producing would be obtained under the conditions as glucose dosage 2.2%,yeast extraction dosage 0.51%,pH value 7.6 and temperature 30.3℃.The strain was also used for the removal of COD in brewery wastewater.Results showed that the best condition of removal of COD was that pH value 8,dosage of 1% CaCl2 in 100mL wastewater as 1.5mL and 2mL respectively.Removal rate of COD under the optimal conditions exceeded 60%.