| 用于有机磷农药残留检测酯酶的筛选 |
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| 引用本文： | 邓光辉,张桂华,孙果宋,黄科林,林润国.用于有机磷农药残留检测酯酶的筛选[J].环境科学与技术,2010,33(12). |
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| 作者姓名： | 邓光辉 张桂华 孙果宋 黄科林 林润国 |
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| 基金项目： | 广西省科学基金项目资助 |
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| 摘 要： | 利用农药对酯酶活力的抑制反应,测定了不同浓度的敌敌畏对游离的不同动物肝脏酯酶的酶活性。实验采用从动物肝脏中提取的粗酶液在水解后与显色剂固兰B盐作用,用分光光度计测定其在595nm处的吸光度。结果表明,猪肝中α-乙酸萘酯酶(α-NA Esterase)的活力较高,且对较低浓度的敌敌畏也有较好的灵敏度。鸡肝和鸭肝α-乙酸萘酯酶对敌敌畏浓度变化不是很明显。
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| 关 键 词： | α-乙酸萘酯酶 有机磷农药 酶抑制法 |
Selection of Esterases Used in Detecting the Residue of Pesticides |
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| DENG Guang-hui,ZHANG Gui-hua,SUN Guo-song,HUANG Ke-lin,LIN Run-guo.Selection of Esterases Used in Detecting the Residue of Pesticides[J].Environmental Science and Technology,2010,33(12). |
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| Authors: | DENG Guang-hui ZHANG Gui-hua SUN Guo-song HUANG Ke-lin LIN Run-guo |
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| Abstract: | Based on the inhibiting mechanism of esterase,the general esterase activities and the specific activities in livers of various animals materials such as chicken liver,duck liver and pork liver were studied and compared for the detection of organophosphate pesticides. Results indicated that α-naphthylacetate hydrolase(α-NA esterase) from pork liver possesses the higher general esterase activity specific,activity and sensitivity,compared to that from chicken liver and duck liver,which makes pork liver an optimal enzyme source for detecting agricultural pesticides residue. |
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| Keywords: | α-naphthylacetate hydrolase (α-NA esterase) organophosphate pesticides enzyme inhibition |
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