生物硝化池污水中硝化细菌的快速定量研究

实验采用聚合酶链式反应（PCR）技术与最大几率数法（MPN）相结合的MPN－PCR法对生物硝化池污水中的硝化细菌进行快速定量。所用的一对PCR引物是在对硝化细菌的16SrRNA基因进行系统比较的基础上设计合成的，可以扩增出大小为388bp的DNA片段。以从生物硝化池污水中抽提的含硝化细菌DNA的混合DNA为模板，进行PCR扩增并确定合适的扩增条件。运用MPN－PCR法进行定量检测的整个过程可在几小时之内完成。

Study on fast quantitative detection for Nitrobacteria in wastewater of biological nitrification stage

The MPN\|PCR method,based on the combination of polymerase chain reaction with most probable number,was applied for quantitative detection of Nitrobacteria in wastewater of biological nitrification stage.The oligonucleotide primers,designed by comparing the total 16SrRNA gene sequences of Nitrobacteria , were used to generate a 388 bp DNA fragment.The mixed DNA (with the Nitrobacteria DNA from the wastewater in the biological nitrification stage) Served as template for PCR and the best amplification condition was determined.It took only several hours to count Nitrobacteria by MPN\|PCR procedure.By comparing the results of MPN\|PCR with that of MPN\|Griess,it was found that MPN\|PCR can sensitively,consistently,efficiently and rapidly assay,which is effective to detect and count the Nitrobacteria in the wastewater of the biological nitrification stage.