颗粒物对滤膜浓缩/密度梯度分离荧光抗体法检测贾第鞭毛虫和隐孢子虫的影响

滤膜浓缩/密度梯度分离荧光抗体法因具有成本低廉和性能稳定的特点,目前已列入《城镇供水水质标准检验方法》(CJ/T 141-2018)用于水源水和城镇供水中贾第鞭毛虫和隐孢子虫(简称两虫)的检测.针对实际生产中,水中颗粒物会影响该方法回收率和稳定性的情况,在前期研究的基础上,结合颗粒物大小,进一步探索了浑浊度对滤膜浓缩/...

Influence of particles on the detection of Giardia and Cryptosporidium by membrane concentration/density gradient separation and immunofluorescence method

Due to its low cost and stable performance, the membrane concentration/density gradient separation and immunofluorescence method has been listed in the Standard of Water Quality Examination Methods for Urban Water Supply (CJ/T 141-2018) for the detection of Giardia and Cryptosporidium in urban supplied water and source water. In view of the fact that particles in water will affect the recovery and stability of this method, based on the previous research and in combination of particle size, the influence of turbidity and particle size on the detection of Giardia and Cryptosporidium in water was further explored. Results showed that particle size contributing to the turbidity of water only affected the recovery of Cryptosporidium, while had no significant effect on the test of Giardia, of which the highest recovery of Cryptosporidium occurred in water mainly containing particles with 5 μm size. Turbidity is the main factor affecting the recovery of this method. The membrane concentration method was preferred to monitor Giardia and Cryptosporidium in water samples with turbidity below 20 NTU, with the best recovery at 3~5 NTU. The sedimentation concentration method was suitable for water samples with turbidity higher than 20 NTU, with the best recovery for Giardia and Cryptosporidium at 30~50 NTU.