|
|||||
|
|
| 环介导等温扩增技术(LAMP)检测人星状病毒方法的建立及其在再生水检测中的应用 | |
| 引用本文： | 杨丽,何晓青,韦玉梅,朱轶,程莉,王子健,黄文.环介导等温扩增技术(LAMP)检测人星状病毒方法的建立及其在再生水检测中的应用[J].生态毒理学报,2011,6(6):600-606. |
| 作者姓名： | 杨丽 何晓青 韦玉梅 朱轶 程莉 王子健 黄文 |
| 作者单位： | 1. 北京林业大学生物科学与技术学院,北京 100083;中国科学院生态环境研究中心环境水质学国家重点实验室,北京 100085;华中农业大学食品科技学院,湖北 武汉 430070 2. 北京林业大学生物科学与技术学院,北京,100083 3. 北京林业大学生物科学与技术学院,北京 100083;中国科学院生态环境研究中心环境水质学国家重点实验室,北京 100085 4. 中国科学院生态环境研究中心环境水质学国家重点实验室,北京,100085 5. 华中农业大学食品科技学院,湖北 武汉,430070 |
| 基金项目： | 国家水体污染控制与治理科技重大专项(2009ZX07527-005)，国家“863”重点项目(2008AA062501)，中国科学院生态环境研究中心环境水质学国家重点实验室开放基金课题(2009－001) |
| 摘 要： | 为快速高效地检测人星状病毒,建立了一种新的试验方法——环介导等温扩增技术(loop-mediated isothermal amplification technology,LAMP),并对人星状病毒LAMP反应条件进行了优化.结果表明,镁离子终浓度为4 mmol·L-1、甜菜碱终浓度为1mol.L-1的25μL体系下... |
| 关 键 词： | 人星状病毒 环介导等温扩增技术(LAMP) HNB 焦磷酸镁 再生水 |
| 收稿时间： | 2011/2/13 0:00:00 |
| 修稿时间： | 5/3/2011 12:00:00 AM |
Loop-Mediated Isothermal Amplification (LAMP) for Detection of Human Astrovirus and Its Application in Reclaimed Water |
|
| Yang Li,He Xiaoqing,Wei Yu-mei,Zhu Yi,Cheng Li,Wang Zijian and Huang Wen.Loop-Mediated Isothermal Amplification (LAMP) for Detection of Human Astrovirus and Its Application in Reclaimed Water[J].Asian Journal of Ecotoxicology,2011,6(6):600-606. | |
| Authors: | Yang Li He Xiaoqing Wei Yu-mei Zhu Yi Cheng Li Wang Zijian and Huang Wen |
| Institution: | 1. School of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083 2. State Key Laboratory of Environmental Aquatic Chemistry, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085 |
| Abstract: | A loop-mediated isothermal amplification(LAMP) technology was developed for rapidly detecting human astrovirus.The data showed that the optimal amplification condition was at 65℃ for 90 min with 4 mmol·L-1 magnesium ions and 1 mol·L-1 betaine.The specificity of RT-LAMP assay was further validated by restriction analgsis of the amplified products and we got two bands(84bp and 135bp) as expected.There is also no cross-reaction with different virus(human rotavirus and norovirus) and restriction analysis of the amplified products.The sensitivity of the LAMP and PCR were 5.04 copies·μL-1 and 5.04×10 copies·μL-1,respectively.Improved RT-LAMP and RT-PCR were both used for detection of human astrovirus in 12 reclaimed water samples.The detection rates of human astrovirus were 41.7%(5/12) and 33.3%(4/12),respectively.All these results suggested that LAMP was a simple,high sensitivity and specificity method which was potential for rapid detection of human astrovirus. |
| Keywords: | human astrovirus LAMP HNB magnesium pyrophosphate reclaimed water |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |
| 点击此处可从《生态毒理学报》浏览原始摘要信息 | |
| 点击此处可从《生态毒理学报》下载免费的PDF全文 | |