Incorporation of pesticides by soil micro-organisms as a way of bound residues formation

We studied the ¹⁴C-tagged biochemical compartments such as proteins, lipids and polysaccharides from bacterial and fungal cultures supplied with ¹⁴C-pesticides and ¹⁴C-glucose. We showed that ¹⁴C incorporation depends both on the substrate structure and on the nature of the micro-organism. Both bacterial and fungal cells incorporated glucose ¹⁴C mainly into cell wall proteins. By contrast, glyphosate ¹⁴C was mainly incorporated into cytoplasm carbohydrates by fungi. On the other hand, 2,4-dichlorophenoxyacetic acid ¹⁴C was mainly found in the carbohydrate fractions of the bacteria walls.