A guideline supplement for determining the aquatic toxicity of poorly water-soluble complex mixtures using water-accommodated fractions

Regulatory guideline methods for aquatic toxicity testing have generally been developed for pure chemicals tested at concentrations below the limit of their water solubility where exposure concentration can be quantified in terms of a concentration in solution. The same methods may also be applied to testing products which are complex chemical mixtures at product: water ratios that do not exceed the water-solubility of any of the product components. A different approach is required for testing complex mixtures at ratios which exceed water-solubilities of some of the components.