高盐度复杂染料废水脱色菌群的降解特性

从天津市市政污泥中筛选出复杂染料脱色菌群，其经16SrDNA基因序列鉴定，主要由Dokdonella（12.26%）、Dyella（10.19%）、Saccharibacteria genera（7.07%）、Rhodobacter（5.80%）、Pseudodoxanthomonas（3.61%）、Flavihumibacter（3.02%）组成.将其用于高盐度复杂染料废水的降解脱色，并研究共代谢基质和各种理化参数对染料废水脱色的影响.结果表明，最佳共代谢基质为葡萄糖和硫酸铵；最佳脱色条件：温度为35℃、pH值为9.0、菌群接种浓度为1.2g/L；在高盐度条件下（50g/L），菌群能高效脱色复杂染料废水，72h脱色率达到（87.0±2.4）%.经高效液相色谱仪（HP-LC）、傅里叶红外光谱（FT-IR）及气相色谱质谱联用仪（GC-MS）分析，甲基橙首先被迅速降解，分散蓝次之，酸性品红的降解较慢；复杂染料的发色基团（-N=N-，-NH_2，-SO₃）和苯环结构被破坏；其降解产物为苯胺、乙酰胺及2-氨基-5-甲基苯甲酸等.

Biodegradation characteristics of high-salinity complex dyes wastewater with high salinity by the bacterial consortium

In this study, a bacterial consortium was isolated from municipal activated sludge from Tianjin, China. Identification via 16SrDNA sequencing showed that it was mainly consisted of Dokdonella (12.26%), Dyella (10.19%), Sacharibacteria genera (7.07%), Rhodobacter (5.80%), Pseudodoxanthomonas (3.61%), and Flavihumibacter (3.02%). The decolorization conditions of high-salinity complex dyes wastewater, such as co-metabolic substrates as well as various physical and chemical parameters, were studied. The results showed that the best co-metabolic substations for the decolorization of complex dyes wastewater were glucose and ammonium sulfate. The optimum decolorization conditions were 35℃, pH 9.0, and inoculum concentration of 1.2g/L. Under the condition of high salinity (50g/L), the decolorization efficiency of complex dyes wastewater reached (87±2.4) % within 72h. Methyl orange was degraded first, followed by disperse blue, while acid fuchsin had a slow degradation rate. The chromophore groups (-N=N-, -NH₂, and -SO₃) and the benzene rings of complex dyes were destroyed, as identified with by high performance liquid chromatography and Fourier transform infrared spectroscopy. Degradation metabolites included aniline, acetamide, and 2-amino-5-methylbenzoic acid as identified by gas chromatography mass spectrometry.