Activity of nucleoside triphosphate diphosphohydrolase, 5′-nucleotidase,and adenosine deaminase in cattle fed on pastures treated with different nitrogen fertilizers |
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Authors: | Juscivete Fátima Favero Ricardo Christ Mateus Eloir Gabriel Luan Cleber Henker Fernanda Agustini Stedille Manoela Marchezan Piva |
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Institution: | 1. Animal Science Graduate Program, Universidade do Estado de Santa Catarina, Chapecó, Brazil;2. Laboratory of Veterinary Pathology, Instituto Federal Catarinense, Concórdia, Brazil |
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Abstract: | The aim of this study was to evaluate nucleoside triphosphate diphosphohydrolase, 5′-nucleotidase, and adenosine deaminase activities in cattle fed on cuttings from pastures fertilized with urea compared to animals fed on cuttings from organically fertilized or from non-fertilized pastures. Blood samples were collected up to 9 h after feeding, and nitrite and enzyme activities were evaluated. The animals feeding on urea-fertilized pasture suffered subclinical intoxication. Serum nitrite levels increased by about 50% and adenosine deaminase activity increased almost sixfold 4 and 6 h after feeding on urea-fertilized pasture, and adenosine deaminase in platelets almost doubled 6 and 9 h after feeding. In platelets, nucleoside triphosphate diphosphohydrolase activity with adenosine triphosphate as substrate increased 2, 4, 6 and 9 h after feeding while nucleoside triphosphate diphosphohydrolase with adenosine diphosphate as substrate and 5′-nucleotidase activities increased only 9 h after feeding. Increased activities of these enzymes may be implicated in the pathophysiology of subclinical intoxication since they are involved in many biochemical functions. |
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Keywords: | ADA ecto-nucleotidases nitrite cattle coagulation disorders |
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