Accumulation of Polyhydroxyalkanoates by Rhizobacteria Underneath Nickel-Hyperaccumulators from Serpentine Ecosystem |
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Authors: | Arundhati Pal A K Paul |
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Institution: | (1) Department of Botany, Serampore College, 9, William Carey Road, Serampore, Hooghly, 712201, India;(2) Microbiology Laboratory, Department of Botany, University of Calcutta, 35, Ballygunge Circular Road, Kolkata, 700019, India; |
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Abstract: | Nickel-resistant bacteria isolated from underneath Ni-hyperaccumulators growing on serpentine soils were screened for production
of polyhydroxyalkanoates. These rhizobacteria accumulated poly-3-hydroxybutyric acid P(3HB)] accounting 3.9–67.7% of cell
dry weight during growth in gluconate and/or glucose. Cupriavidus pauculus KPS 201 utilized only gluconate and accumulated about 67.7% P(3HB) while, Bacillus firmus AND 408 utilized both carbon sources for polymer synthesis. The isolates being resistant to Ni also accumulated substantial
amount of P(3HB) when grown in presence of the heavy metal and this was revealed by transmission electron microscopic studies.
Although B. firmus AND 408 produced only P(3HB) at higher concentrations of gluconate, C. pauculus KPS 201 synthesized copolymer of 3-hydroxybutyric acid (3HB) and 3-hydroxyvaleric acid (3HV) P(3HB-co-3HV)]. In presence
of 0.8% gluconate and 4 mM Ni, KPS 201 cells produced PHA amounting 81% CDW, which contained 76 and 24 mol% 3HB and 3HV monomers,
respectively. |
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