| 海绵体上具有抗肿瘤活性的细菌B2817的分离与鉴定 |
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| 引用本文： | 吕家森,黄惠琴,丛明,鲍时翔,符健.海绵体上具有抗肿瘤活性的细菌B2817的分离与鉴定[J].应用与环境生物学报,2005,11(5):588-591. |
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| 作者姓名： | 吕家森 黄惠琴 丛明 鲍时翔 符健 |
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| 作者单位： | 1. 中国热带农业科学院生物技术国家重点实验室
2. 海南医学院药理教研室,海口,571101 |
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| 基金项目： | 国家自然科学基金(No.30460152)，海南省教育厅科研基金(HJKJ200318)，中国热带农业科学院科技基金资助~~ |
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| 摘 要： | 建立了镰刀菌-细胞毒组合筛选模型,并运用该模型对232株从海绵体上分离出的细菌进行筛选,得到4株抗肿瘤活性较好的细菌.经活性检测,发现菌株B2817的发酵液在稀释100倍时对人肝癌细胞SMMC-7721、人鼻咽癌细胞CNE和小鼠肉瘤细胞S180的抑制率分别为92%、90%和95%,而对正常的人肝细胞的抑制率仅为8%.经多相分类学鉴定,发现B2817是弧菌属中一个种,与弧菌属中的产气弧菌Vibriogazogenes亲缘关系最近,但两者在形态、生理生化特征、分子遗传等方面存在很大差异,故认为B2817可能是弧菌属的一株未被认识的新种.图1表3参9
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| 关 键 词： | 海绵 分离 抗肿瘤 弧菌属 |
| 收稿时间： | 2004-08-23 |
| 修稿时间： | 2005-10-21 |
ISOLATION AND IDENTIFICATION OF STRAIN B2817 WITH CYTOTOXIC ACTIVITY IN SPONGE |
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| L Jiasen,HUANG Huiqin,CONG Ming,BAO Shixiang,FU Jian.ISOLATION AND IDENTIFICATION OF STRAIN B2817 WITH CYTOTOXIC ACTIVITY IN SPONGE[J].Chinese Journal of Applied and Environmental Biology,2005,11(5):588-591. |
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| Authors: | L Jiasen HUANG Huiqin CONG Ming BAO Shixiang FU Jian |
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| Institution: | 1. State Key Laboratory of Biotechnology, Chinese Academy for Tropical Agriculture Sciences, Haikou 571101, China; 2. Laboratory of Pharmacology, Hainan Medical College, Haikou 571101, China |
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| Abstract: | A combinatorial model for screening marine microorganisms with anti-tumor activity was established. By using this model, four strains with anti-tumor activity were isolated from 232 bacterial strains in sponge. The inhibitory rates of fermentation broth of strain B2817 diluted 100 times against human liver cancer cell SMMC-7721, rat sarcoma cell S180, nasopharyngeal cancer cell CNE were 92%, 90% and 95%, respectively, but the inhibitory rate against normal liver cell L-20 was only 8%. Numerical analysis indicated that B2817 was a species of genus Vibrio and shared a high homology with V. gazogenes. However, there were obvious differences between them in phenotypic and chemotaxonomic characterization. The result from this study indicates that B2817 is probably a novel species of Vibrio. Fig 1, Tab 3, Ref 9 |
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| Keywords: | sponge isolation antitumor Vibrio |
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