Noncovalent immobilization of manganese peroxidases from P. chrysosporium on carbon nanotubes |
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| Authors: | Jiaxi Li and Xianghua Wen |
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| Affiliation: | (1) Department of Applied Chemistry and Microbiology, Division of Microbiology, University of Helsinki, Viikki Biocenter 1, P.O.Box 56, 00014 Helsinki, Finland; |
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| Abstract: | Manganese peroxidases (MnP) from Phanerochaete chrysosporium were adsorbed onto multi-walled carbon nanotubes (MWNT). Four different loadings of MnP on MWNTs were investigated, and the maximum enzyme loading of 47.5 μg/mg of MWNTs was obtained in 12 h. The adsorbed MnP showed a catalytic activity of up to 0.1 U/mg of the weight of the system of MnP/MWNTs, with 23% of its original activity retained. The AFM image of the adsorbed enzymes indicated that a layer of MnP covered the surface of the MWNTs and retained its original three-dimensional shape. Amino-based nonspecific interactions may play the dominant role in the adsorption of MnP on MWNTs. |
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