Development,validation and matrix effect of a QuEChERS method for the analysis of organochlorine pesticides in fish tissue |
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| Authors: | Tatiana R De O Stremel Cinthia E Domingues Rosimara Zittel Cleber P Silva Patricia L Weinert Franciele C Monteiro |
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| Institution: | 1. Department of Chemistry, Research Group on Environmental and Sanitary Analytical Chemistry (QAAS), Ponta Grossa State University (UEPG), Ponta Grossa, Paraná, Brazil;2. Department of Chemistry, Ponta Grossa State University (UEPG), Ponta Grossa, Paraná, Brazil;3. Department of Chemistry, Graduate Program in Chemistry, Ponta Grossa State University (UEPG), Ponta Grossa, Paraná, Brazil |
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| Abstract: | This study aims to develop and validate a method to determine OCPs in fish tissues, minimizing the consumption of sample and reagents, by using a modified QuEChERS along with ultrasound, d-SPE and gas chromatography with an electron capture detector (GC-ECD), refraining the pooling. Different factorial designs were employed to optimize the sample preparation phase. The validation method presented a recovery of around 77.3% and 110.8%, with RSD lower than 13% and the detection limits were between 0.24 and 2.88 μgkg?1, revealing good sensitiveness and accuracy. The method was satisfactorily applied to the analysis of tissues from different species of fish and OCPs residues were detected. The proposed method was shown effective to determine OCPs low concentrations in fish tissues, using small sample mass (0.5 g), making the sample analyses viable without the need for grouping (pool). |
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| Keywords: | QuEChERS organochlorine pesticides fish tissue GC-ECD |
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