纤维素降解真菌的分离鉴定及特性研究

经过实验分离到一株纤维素降解真菌CD-Q1,通过形态及分子生物学鉴定该真菌为匍匐根霉。该菌能够以滤纸、秸秆和脱酯棉为唯一碳源生长,8 d内可将26.5%的秸秆降解,可将7.5%的滤纸糖化。当以滤纸为唯一碳源时,pH=5条件下,滤纸失重及还原糖产生量最高,45 h内可将3%滤纸降解,生成0.845 mg/ml的还原糖(以葡萄糖计)。该纤维素降解真菌的分离为纤维素糖化及进一步利用提供了较好菌源,为纤维素降解性能的基因改良提供了理想的出发菌株。

Isolation,Identification and Characteristics of A Cellulose-degradation Fungus

A cellulose-degradation fungus CD-Q1 was isolated and further determined as Rhizo-pus stolonifer by morphology and molecular biological technique. This fungus is able to grow with filter paper, taking straw or ester cotton as sole carbon source. It is capable to decompose 26.5%straw, 7.5% filter paper in 8 d. When using filter paper as sole carbon source, it can produce maximum reducing sugar when pH=5 and decompose 3% filter paper in 45 h and obtain 0.845 mg/ml of reducing sugar （as glucose）. This study provided a fine seed for cellulose-degradation and for further genetic modification.