Dietary bioflavonoid quercetin modulates porcine ovarian granulosa cell functions in vitro |
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| Authors: | Adriana Kolesarova Shubhadeep Roychoudhury Bibiana Klinerova Dagmara Packova Katarina Michalcova Marek Halenar |
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| Institution: | 1. Department of Animal Physiology, Faculty of Biotechnology and Food Sciences, Slovak University of Agriculture in Nitra, Nitra, Slovak Republic;2. Department of Life Science and Bioinformatics, Assam University, Silchar, India;3. The Jessenius Faculty of Medicine, Comenius University in Bratislava, Bratislava, Slovak Republic |
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| Abstract: | Quercetin is a dietary bioflavonoid used widely as a food supplement and is generally recognized as safe. The aim of this in vitro study was to examine the steroid hormone (progesterone and 17- β estradiol) release, proliferation (PCNA and cyclin B1) and apoptosis (caspase 3 and p53) of porcine ovarian granulosa cells after the addition of quercetin at concentrations 0.01, 0.1, 1, 10 and 100?μmol?L?1. Progesterone release was stimulated at the concentration 10?μmol?L?1. Quercetin neither had any impact on 17-β estradiol secretion nor on the presence of PCNA. However, a significant enhancement of the occurrence of cyclin B1 was noted except for the lowest concentration 0.01?μmol?L?1. Quercetin did not have any influence on the number of granulosa cells containing caspase 3, but at the concentration 10?μmol?L?1 it inhibited p53 occurrence. Results confirm the safety of quercetin in porcine ovarian granulosa cell model and further suggest its possible concentration-dependent influence on ovarian functions through pathway that may involve progesterone, cyclin B1 and p53. |
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| Keywords: | Quercetin ovarian functions progesterone 17β-estradiol proliferation apoptosis |
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