异化Mn(IV)还原菌激活及其对有机药物去除

以嘉陵江沉积物为菌源，利用荧光绝对定量（qPCR）技术探究了不同碳源（葡萄糖和乙酸钠）和不同形态的锰氧化物（δ-MnO₂和锰矿粉）对异化Mn（IV）还原菌激活效果的影响.在此基础上，研究了激活效果最佳的异化Mn（IV）还原菌对卡马西平、布洛芬、萘普生、雌激素和双氯芬酸5种有机药物的去除效果.结果表明，葡萄糖作为碳源，δ-MnO₂作为电子受体时激活效果最佳，Mn²⁺10d累计生成浓度达416.03mg/L，TOC消耗率达88.24%.激活后的异化金属还原菌在有无外加碳源时对卡马西平和布洛芬均无明显去除，对萘普生在外加碳源时能实现11.88%的去除.雌二醇和双氯芬酸可以作为异化Mn（IV）还原菌唯一碳源，其去除率可达75.70%和58.25%.

Activation of dissimilatory Mn(IV) reducing bacteria and their removal of typical pharmaceuticals

In this study, sediment from JiaLing River was cultivated as inoculum of dissimilatory Mn(IV)-reducing bacteria. By using qPCR technique, this study investigated the effects of different carbon sources (glucose and Na-acetate) and different forms of manganese oxides (δ-MnO₂ and manganese ore powders) on activating dissimilatory Mn(IV)-reducing bacteria. Moreover, the most active dissimilatory Mn(IV) reducing bacteria was further tested the performance of removing 5 typical pharmaceuticals, namely carbamazepine, ibuprofen, naproxen, estradiol and diclofenac. Results demonstrated that glucose was the most suitable carbon sources while the δ-MnO₂ was the most suitable electron acceptor for the activation of dissimilatory Mn(IV)-reducing bacteria. The accumulated concentration of Mn²⁺ reached 416.03mg/L after 10d cultivation, while the total consumption of TOC was 88.24%. After 5d cultivation, removal of carbamazepine and ibuprofen by the activated dissimilatory Mn(IV)-reducing bacteria was insufficient with or without additional carbon sources. Only 11.88% of naproxen was removed by the bacteria in the presence of additional carbon sources. However, the activated dissimilatory Mn(IV)-reducing bacteria could use estradiol and diclofenac as the sole carbon source, with removal efficiency of 75.70% and 58.25%, respectively.