氯苯对斑马鱼胚胎发育和仔鱼的毒性效应研究

选取健康的雌雄斑马鱼按1:1或1:2的比例进行交配产卵。采用静态法,以丙酮为助溶剂,分别用不同浓度的CB进行斑马鱼受精卵和刚平衡游动仔鱼的暴露试验,记录胚胎孵化数和胚胎、仔鱼畸形数以及仔鱼死亡数。不同浓度的CB可导致斑马鱼胚胎孵化率降低及孵出的仔鱼畸形率增加,且毒性呈现剂量-效应关系。当CB浓度高于0.88μg/L时,斑马鱼胚胎及其孵出的仔鱼畸形率开始增加;当CB为108.9μg/L时,暴露24 h的胚胎出现全致畸效应。能平衡游动的斑马仔鱼对CB的毒性也很敏感,24 h、72 h和96 h的LC50值分别为95.35、80.51和62.28μg/L;110.0μg/L CB使处理24 h的仔鱼全部死亡。较低浓度的CB就使斑马鱼胚胎发育畸形;其对仔鱼的毒性也很显著,达到一定浓度可使其死亡。

Toxic Effects of Chlorobenzene on Embryonic Development and Larva of Zebrafish

Toxicity of chlorobenzene(CB)on the embryonic development and larva of zebrafish were studied.Healthy male and female zebrafishes were selected to mate and oviposit in the proportion of 1:1 or 1:2.The exposure test of CB on fertilized eggs and just balance-swimming larva of zebrafish was made with static method,and acetone was used as cosolvent.Numbers of embryonic hatching,embryo and larval abnormality and larval death were counted.Results indicated that CB of different concentrations could reduce the embryonic hatching rate and increase the hatched larval deformity rate,and toxic effect was dose-dependent.When CB concentration was higher than 0.88 μg/L,malformation rate of zebrafish embryos and hatched larval began to increase.As for CB 108.9 μg/L,teratogenic effect was found for all embryos after 24 h.The just balance-swimming zebrafish larva was sensitive to CB,and LC50of 24 h,72 h,96 h was 95.35,80.51 and 62.28 μg/L respectively.When CB concentration was 110.0 μg/L,all larvae were dead during 24 h.CB was toxic on zebrafish embryonic development even at lower concentrations.Toxicity of CB to zebrafish larva was remarkable,when CB concentration reached a certain extent,it could cause the zebrafish larva to death.