表面展示技术在污染环境生物修复中的应用

革兰氏阴性细菌、革兰氏阳性细菌和酵母中已建立多个表面展示短肽和蛋白质的系统.在微生物细胞表面展示外源蛋白对污染环境的生物修复有着重要的意义.展示金属结合蛋白(肽)的微生物可用于污染土壤和工业废水的净化,展示有机磷水解酶的微生物将用于有机磷污染物的脱毒.微生物表面展示技术将成为污染环境生物修复的有效策略.这一技术的研究还是一个新领域,要使其得到应用还有许多问题需要解决. 参38

APPLICATIONS OF CELL- SURFACE DISPLAY TECHNOLOGY IN BIOREMEDIATION OF POLLUTED ENVIRONMENTS

Many systems for the display of either short peptides or native proteins on Gram-negative bacteria, Gram-positive bacteria and yeast have been developed. The expression of heterologous proteins on the surface of microbial cells is proved very important for bioremediation. The microbes, expressing metal-binding peptide/protein on cell surface, can be used as bioadsorbents in the purification of contaminated soil and industrial wastewater while those displaying organophosphorus hydrolase are to be used for detoxification of organophosphorus contaminants. Surface display of engineered peptide/protein on microbes might become a useful strategy for the effective bioremediation of polluted environments, but the corresponding research is still a relatively new field and some problems need to be addressed before application. Ref 38