The Analysis of Tecnazene (2,3,5,6-Tetrachloronitro-benzene) and its Metabolites in Water and Fish

A method for the analysis of Tecnazene (2,3,5,6-tetrachloronitrobenzene) and its major metabolites (2,3,5,6-tetrachloroaniline and 2,3,5,6-tetrachlorothioanisole) is presented. This rapid analytical technique was developed to measure these compounds in samples of water and fish produced during the course of toxicological investigations. Water samples were extracted into hexane and analysed by high resolution capillary gas chromatography using flame ionisation detection (HRGC-FID). Fish samples were extracted into hexane, cleaned up on a short alumina micro-column and also analysed by HRGC-FID.