复配逆胶束体系中漆酶催化性能研究

在由鼠李糖脂和Tween-80构建而成的复配逆胶束媒介研究了漆酶的催化性能.采用紫外法考察了Tween-80摩尔含量、逆胶束含水量、水相酸碱度、水相盐度以及助表面活性剂种类等反应条件对漆酶催化性能的影响.研究结果表明,反应的最佳条件:Tween-80的摩尔含量为30%,体系含水量为20,缓冲溶液pH为4.5,KCI浓度为70mmol/L,助表面活性剂正己醇.并且在最佳条件下,漆酶在复配逆胶束体系中的酶活比在单相鼠李糖脂逆胶束中高1.44倍,比在水溶液中高4.35倍,研究结果表明复配表面活性剂在构建胶束酶学上具有很大的潜力.

Catalytic performance of laccase in mixed reversed micelle system

A mixed reversed micelle system had been developed to investigate the catalytic performance of laccase. The anionic biosurfactant Rhamnolipid and nonionic surfactant Tween-80 were used to construct the mixed reversed micelle system, while the isooctane and short-chain alcohols (1:1, V/V) were used as oil phase and cosurfactant, respectively. UV spectrum was analysed to find out how the molar fraction Tween-80, water content, pH, ionic strength and cosurfactant stype affected enzyme activity of laccase in the mixed reversed micelle system. And it has been found that while the molar fraction Tween-80 was 30%, water content was 20, pH was 4.5, the concentration of KCl was 70mmol/L, cosurfactant stype was n-hexanol, the optimal condition of mixed reversed micelle system could be reached. In optimum catalytic conditions, the catalytic activity was higher 1.44 times in the mixed reversed micelle than that in the single Rhamnolipid reversed micelle system, and higher 4.35 times than that in water. The research indicated mixed reversed micelle has huge potential in micellar enzymology.