同步硝化反硝化系统中反硝化细菌多样性研究

采用聚合酶链式反应(PCR)和分子克隆构建nirS克隆文库对同步硝化反硝化系统好氧池中反硝化细菌多样性进行了研究.从克隆文库中随机挑选75个克隆子进行序列测定,对测序结果进行了BLAST比对.结果表明,有74个克隆子分属于3个不同的细菌类群,包括β-Proteobacteria、γ-Proteobacteria和Uncultured bacterium. β-Proteobacteria纲为好氧池内优势菌群,占文库比例的54.41%;其次是γ-Proteobacteria纲,占文库比例的25%.对测序得到的12个OTU用MEGA软件进行系统发育分析,结果显示Thauera属为该系统中最主要的脱氮菌属.

Analysis of denitrifying bacteria community composition in simultaneous nitrification and denitrification systems

The diversity of denitrifying bacteria in the aerobic tank of a simultaneous nitrification and denitrification system was studied by building nirS cloning library chain reaction (PCR) and molecular cloning. 75 clones were randomly selected from the cloning library to be sequenced and blasted. 74 clones belonged to three different bacteria groups, including β-proteobacteria、γ-proteobacteria and uncultured bacterium. β-proteobacteria was the dominant bacterial group in the aerobic tank, which accounted for 54.41% in the library; γ-proteobacteria was the second group and accounted for 25%. 12 OTU were selected from the 74 sequenced clones for phylogenetic analysis using MEGA software, the Thauera was the main denitrifier genera in the system.