Biomineralization markers during early shell formation in the European abalone <Emphasis Type="Italic">Haliotis tuberculata</Emphasis>, Linnaeus |
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Authors: | Béatrice Gaume Martine Fouchereau-Peron Aïcha Badou Marie-Noëlle Helléouet Sylvain Huchette Stéphanie Auzoux-Bordenave |
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Institution: | 1.UMR BOREA (Biologie des Organismes et Ecosystèmes Aquatiques), MNHN/CNRS 7208/IRD 207/UPMC,Muséum national d’Histoire naturelle,Concarneau,France;2.UMR 7194/USM 204, Centre de Spectroscopie Infrarouge, Département Préhistoire,Muséum National d’Histoire Naturelle,Paris,France;3.Ecloserie France-Haliotis,Plouguerneau,France;4.Université Pierre et Marie Curie Paris VI,Paris,France |
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Abstract: | Larval shell formation was investigated in the European abalone Haliotis tuberculata. Stages of mineralization as well as enzymatic and endocrine biomarkers were monitored throughout larval development, from
hatching to post-larval stages. Polarized light microscopy and infrared spectroscopy analyses revealed the presence of crystallized
calcium carbonate arranged in aragonite polymorphs from the late trochophore stage. A correlation between the main steps of
shell formation and enzymatic activities of alkaline phosphatase and carbonic anhydrase was seen. The variations of these
biologic activities were related to the onset of mineralization, the rapid shell growth, and the switch from larval to juvenile
shell following metamorphosis. Furthermore, a strong increase in the level of calcitonin gene-related molecules was measured
in post-larvae, suggesting that endocrine control takes place after metamorphosis. The changes measured for the three biomineralization
markers together with mineralogical analysis allowed us to correlate physiologic mechanisms with early steps of abalone shell
formation. |
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