甲醛对小鼠骨髓组织的毒性作用

为了探讨甲醛暴露对小鼠骨髓组织的毒性影响,选用SPF级昆明雄性小鼠作为研究对象,用浓度为0.5,1.0,3.0mg/m3的气态甲醛对小鼠进行连续动态染毒72h,染毒后检测骨髓细胞中细胞周期变化、DPC(DNA-蛋白质交联)、DNA稳定性、骨髓细胞分化关键因子Nucleostemin和CYP1B1在各暴露浓度组中的表达情况.结果表明:甲醛暴露造成骨髓细胞的DNA损伤,其DNA稳定性和DPC效应均呈现良好的剂量-效应关系.骨髓细胞分化关键因子Nucleostemin表达量在各浓度染毒组中与空白组相比,1.0mg/m3浓度组有极显著性升高,而0.5和3.0mg/m3浓度组则是极显著性降低,与空白组相比CYP1B1基因在0.5,1.0,3.0mg/m3浓度组中表达,有极其显著性差异,并且1.0mg/m3浓度组上升较多.本次研究表明,甲醛暴露能影响小鼠骨髓组织细胞正常生长代谢.

Toxic effect of formaldehyde on mouse bone marrow

An investigation on the toxic effect of formaldehyde on bone marrow of male SPF level Kunming mice was conducted.After a 72h continuous and dynamic exposure to gaseous formaldehyde with three different concentrations(0.5mg/m3,1.0mg/m3 and 3.0mg/m3,respectively),cell number at different phases of the cell cycle,DNA-protein crosslink(DPC),DNA stability and the expression profile of Nucleostemin and CYP1B1,two key factors in bone marrow cell differentiation were detected.The results indicated that formaldehyde exposure could cause DNA damage of bone marrow cell,with an obvious dose-response relationship between the DNA stability and DPC effect.Compared with the control group,the expression of Nucleostemin significantly increased in the 1.0mg/m3 group while it significantly decreased in the 0.5 and 3.0mg/m3 groups.The expression of CYP1B1 was also significantly different with that in the control group and the most obvious increase was found in the 1.0mg/m3 group.Thereby we conclude that mice exposed to formaldehyde can affect normal growth and metabolism of bone marrow cells.