| 喹啉为MFC阳极燃料的微生物群落结构演化分析 |
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| 引用本文： | 张翠萍,谢健,贾后磊,李明臣,罗勇,李婕. 喹啉为MFC阳极燃料的微生物群落结构演化分析[J]. 中国环境科学, 2012, 32(6): 1093-1097 |
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| 作者姓名： | 张翠萍 谢健 贾后磊 李明臣 罗勇 李婕 |
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| 作者单位： | 1. 国家海洋局南海海洋工程勘察与环境研究院,广东广州,510300
2. 中山大学环境科学与工程学院,广东广州,510275 |
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| 基金项目： | 国家自然科学基金资助项目，环境模拟与污染控制国家重点联合实验室专项基金 |
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| 摘 要： | 通过构建填料型微生物燃料电池(MFC),首次对以喹啉为燃料时的MFC阳极表面的微生物群落进行了分析.PCR-DGGE的试验结果表明,随着燃料的改变,微生物群落也发生改变.当以喹啉和葡萄糖的混合溶液稳定地作为燃料时,由于受到喹啉毒性的抑制,微生物多样性降低,优势菌也发生明显的改变.与葡萄糖共基质相比,以单一喹啉为燃料时的阳极微生物优势菌落发生明显改变.新增加一类菌,这类菌与Pseudomonas sp. DIC5RS 的同源性为100%,推测该菌在单一喹啉为MFC燃料时喹啉的降解过程中起到关键作用.
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| 关 键 词： | 喹啉 微生物燃料电池 微生物群落演化 |
| 收稿时间： | 2011-09-24; |
Microbial community analysis of microbial fuel cells fueled with quinoline |
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| ZHANG Cui-ping , XIE Jian , JIA Hou-lei , LI Ming-chen , LUO Yong , LI Jie. Microbial community analysis of microbial fuel cells fueled with quinoline[J]. China Environmental Science, 2012, 32(6): 1093-1097 |
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| Authors: | ZHANG Cui-ping XIE Jian JIA Hou-lei LI Ming-chen LUO Yong LI Jie |
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| Affiliation: | 1.South China Sea Marine Engineering and Environment Institute,Guangzhou 510300,China;2.School of Environmental Science and Engineering,Sun Yat-sen University,Guangzhou 510275,China) |
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| Abstract: | A graphite-packed MFC was constructed to investigate anode microbial community firstly in the MFC when using quinoline as fuel.PCR-DGGE analysis showed that microbial community changed with the substrates.Due to the inhibition of quinoline toxic ability,microbial species diversity decreased and the dominating species changed significantly when using quinoline-glucose mixture.Compared to the mixed substrates with glucose,the microbial community changed significantly when the substrate changed to sole quinoline.Furthermore,a new species was found,which had the highest homology(100%) with Pseudomonas sp.DIC5RS.We speculated that this strain played the key role in degradating quinoline when using sole quinoline as the fuel. |
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| Keywords: | quinoline microbial fuel cells(MFC) microbial community analysis |
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