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臭氧水杀灭模式微生物效果的试验研究
引用本文:鲁春.臭氧水杀灭模式微生物效果的试验研究[J].环境科学与管理,2011,36(9):82-85.
作者姓名:鲁春
作者单位:镇江市环境保护局,江苏镇江,212010
摘    要:臭氧水对所选择的模式生物(大肠杆菌、不动杆菌)均有很强的杀灭作用。随着施加臭氧水剂量(浓度)的上升,模式生物的灭活率显著上升;在1 mL的大肠杆菌菌液中滴加2 500μL臭氧水就可以将浓度约为106 CFU/mL的大肠杆菌全部杀灭;在1mL的不动杆菌菌液中滴加1 750μL臭氧水就可以将浓度约为106CFU/mL的不动杆菌全部杀灭,而且杀灭细菌效果持久,均无细菌再生现象。大肠杆菌对于高浓度臭氧水的抗性大于不动杆菌,其灭活速率K值分别为0.192 min-1、0.279 9 min-1,Chick一级反应动力学模型能够体现出大肠杆菌、不动杆菌的灭活特性,所得结论与实验结果一致。

关 键 词:臭氧水  杀菌  大肠杆菌  不动杆菌  剂-效关系

Experimental Investigation on Sterilization Effect of High Concentration Ozone Water
Lu Chun.Experimental Investigation on Sterilization Effect of High Concentration Ozone Water[J].Environmental Science and Management,2011,36(9):82-85.
Authors:Lu Chun
Institution:Lu Chun (Zhenjiang Environmental Protection Agency, Zhenjiang 212010, China)
Abstract:High concentration ozone water has high efficacy in killing the selected model organisms ( E. cob, Acinetobacter). With the increasing of applied ozone water dose ( concentration), the inactivation rate of model organisms increased significantly : 1 ml of E. coli bacteria liquid( 10^6 CFU/ml) can be completely killed with 2 500 μL ozone water; lml of Acinetobacter bacteria liquid( 10^6 CFU/ml) can be completely killed with 1 750 μL ozone water. The sterilizing effect is durable and no bacteria regeneratiQn was detected. E. Coli had stronger resistance capability to high concentration ozone water than Acinetobacter, and the inactivation rate were 0. 192 min^-1 and 0. 279 9 min^- 1, respectively. The first order reaction dynamic model of Chick Model could embody the inactivation feature of E. coli and acinetobacter and the conclusion agreed with experimental results.
Keywords:ozone water  sterilize  E  coli  acinetobacter  dose - dependent manner
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