| 氯氰菊酯降解菌CY22-7的分离鉴定及降解特性研究 |
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| 引用本文： | 崔志峰,汪华,王渭霞,汪琨,朱廷恒. 氯氰菊酯降解菌CY22-7的分离鉴定及降解特性研究[J]. 环境污染与防治, 2009, 31(11) |
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| 作者姓名： | 崔志峰 汪华 王渭霞 汪琨 朱廷恒 |
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| 作者单位： | 浙江工业大学生物与环境工程学院,浙江,杭州,310032;中国水稻研究所,浙江,杭州,310006 |
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| 基金项目： | 浙江工业大学重中之重学科开放研究基金资助项目 |
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| 摘 要： | 从采集的多种土壤样品中.获得了19株在3种筛选平板上均生长良好的候选氯氰菊酯降解菌.通过生理生化特性鉴定和16S rDNA序列的测定及比对.将其中的氯氰菊酯降解菌CY22-7鉴定为中华根瘤菌属(Sinorhizobium sp.).同时,对氯氰菊酯降解菌CY22-7的降解特性研究结果表明:(1)氯氰菊酯降解菌CY22-7以5%(体积分数)的接种鼍接种到氯氰菊酯起始质量浓度为100 mg/L的氯氰菊酯乙醇培养基后,于200 r/min、30℃摇床中培养.经过6 d的培养,氯氰菊酯降解菌CY22-7降解了约60%的氯氰菊酯.(2)加人外源营养物质有利于促进氯氰菊酯的降解.其中,葡萄糖和酵母提取物的促进作用最为明显.(3)氯氰菊酯降解菌CY22-7降解氯氰菊酯的最适温度为30℃,最佳pH为6.0.
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| 关 键 词： | 氯氰菊酯 生物降解 中华根瘤菌属 |
Isolation and characterization of a new cypermethrin degrading bacterium CY22-7 |
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| Cui Zhifeng,Wang Hua,Wang Weixia,Wang Kun,Zhu Tingheng. Isolation and characterization of a new cypermethrin degrading bacterium CY22-7[J]. Environmental Pollution & Control, 2009, 31(11) |
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| Authors: | Cui Zhifeng Wang Hua Wang Weixia Wang Kun Zhu Tingheng |
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| Abstract: | A cypermethrin-degrading bacterium named CY22-7 was isolated from pesticide contaminated soils. This bacterium was identified as Sinorhizobium sp. according to its physiological and biochemical characteristics and the similarity analysis of its 16S rDNA sequence. Under the inorganic salt medium with the bacterium inoculation of 5% ,60% of 100 mg/L cypermethrin was degraded by this strain in 6 d at 30℃. The effects of initial pesticide con-centration,added nutriment, temperature, and pH on cypermethrin degrading by CY22-7 was also investigated. The presence of glucose and yeast extract were beneficial for cypermethrin degrading. |
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| Keywords: | cypermethrin biodegradation Sinorhizobium sp |
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