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Comparison of endotoxin levels and gram-negative bacteria under different conditions in microbial laboratories and a biowaste site |
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| Authors: | Hwang Sung Ho Park Dong Uk Joo Se Ik Park Hyun Hee Yoon Chung Sik |
| Institution: | a Institute of Health and Environment, School of Public Health, Seoul National University, Gwanak 599, Gwanak-ro, Seoul 151-742, South Korea b Department of Environmental Health, Korea National Open University, Dongsung-Dong Jongro-Gu, Seoul 110-791, South Korea c Department of Laboratory Medicine, Seoul National University Hospital, 28 Yunkun-Dong Jongro-Gu, Seoul 110-744, South Korea d Occupational Safety and Health Research Institute, Korea Occupational Safety and Health Agency, 34-4 Guasn-Dong Bupyeong-Gu, Incheon 403-711, South Korea |
| Abstract: | In this study, we assessed airborne endotoxin levels in university laboratories, hospital diagnostic laboratories, and a biowaste site. We also investigated indoor and outdoor sampling, sampling site, type of ventilation system, presence of open biowaste boxes, weather, and detection of Gram-negative bacteria (GNB). A total of 69 air samples were collected from 11 facilities in three institutions. Average total airborne endotoxin levels ranged from <0.01 to 10.02 EU m−3, with an overall mean of 1.03 EU m−3. Endotoxin levels were high in window-ventilated facilities, in facilities in which GNB were detected; levels were also high when it was rainy (all ps < 0.05). Endotoxin levels were significantly correlated with humidity (r = 0.70, p < 0.01). The presence of HVAC; humidity; and the presence of open biowaste boxes affect endotoxin levels in laboratories. |
| Keywords: | Airborne endotoxin Laboratory Gram-negative bacteria Ventilation |
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